Universität zu Köln

Hackl, Matthias J., Burford, James L., Villanueva, Katie, Lam, Lisa, Susztak, Katalin ORCID: 0000-0002-1005-3726, Schermer, Bernhard ORCID: 0000-0002-5194-9000, Benzing, Thomas and Peti-Peterdi, Janos ORCID: 0000-0003-4092-6185 (2013). Tracking the fate of glomerular epithelial cells in vivo using serial multiphoton imaging in new mouse models with fluorescent lineage tags. Nat. Med., 19 (12). S. 1661 - 1667. NEW YORK: NATURE PUBLISHING GROUP. ISSN 1546-170X

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Abstract

Podocytes are critical in the maintenance of a healthy glomerular filter; however, they have been difficult to study in the intact kidney because of technical limitations. Here we report the development of serial multiphoton microscopy (MPM) of the same glomeruli over several days to visualize the motility of podocytes and parietal epithelial cells (PECs) in vivo. In podocin-GFP mice, podocytes formed sporadic multicellular clusters after unilateral ureteral ligation and migrated into the parietal Bowman's capsule. The tracking of single cells in podocin-confetti mice featuring cell-specific expression of CFP, GFP, YFP or RFP revealed the simultaneous migration of multiple podocytes. In phosphoenolpyruvate carboxykinase (PEPCK)-GFP mice, serial MPM found PEC-to-podocyte migration and nanotubule connections. Our data support a highly dynamic rather than a static nature of the glomerular environment and cellular composition. Future application of this new approach should advance our understanding of the mechanisms of glomerular injury and regeneration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hackl, Matthias J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Burford, James L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Villanueva, Katie UNSPECIFIED UNSPECIFIED UNSPECIFIED Lam, Lisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Susztak, Katalin UNSPECIFIED orcid.org/0000-0002-1005-3726 UNSPECIFIED Schermer, Bernhard UNSPECIFIED orcid.org/0000-0002-5194-9000 UNSPECIFIED Benzing, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Peti-Peterdi, Janos UNSPECIFIED orcid.org/0000-0003-4092-6185 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-470910
DOI:	10.1038/nm.3405
Journal or Publication Title:	Nat. Med.
Volume:	19
Number:	12
Page Range:	S. 1661 - 1667
Date:	2013
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	NEW YORK
ISSN:	1546-170X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language URETERAL OBSTRUCTION; ATUBULAR GLOMERULI; NEPHROTIC SYNDROME; PODOCYTES; INJURY; MICE; RECOMBINASE; RECRUITMENT; PROTEINURIA; REVEALS Multiple languages Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47091