Acevedo-Garcia, Johanna, Collins, Nicholas C., Ahmadinejad, Nahal, Ma, Lu ORCID: 0000-0001-5271-0686, Houben, Andreas ORCID: 0000-0003-3419-239X, Bednarek, Pawel ORCID: 0000-0002-3064-7775, Benjdia, Mariam ORCID: 0000-0003-1554-5452, Freialdenhoven, Andreas, Altmueller, Janine, Nuernberg, Peter, Reinhardt, Richard ORCID: 0000-0001-9376-2132, Schulze-Lefert, Paul and Panstruga, Ralph ORCID: 0000-0002-3756-8957 (2013). Fine mapping and chromosome walking towards the Ror1 locus in barley (Hordeum vulgare L.). Theor. Appl. Genet., 126 (12). S. 2969 - 2983. NEW YORK: SPRINGER. ISSN 1432-2242

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Abstract

The Ror1 gene was fine-mapped to the pericentric region of barley chromosome 1HL. Recessively inherited loss-of-function alleles of the barley (Hordeum vulgare) Mildew resistance locus o (Mlo) gene confer durable broad-spectrum disease resistance against the obligate biotrophic fungal powdery mildew pathogen Blumeria graminis f.sp. hordei. Previous genetic analyses revealed two barley genes, Ror1 and Ror2, that are Required for mlo-specified resistance and basal defence. While Ror2 was cloned and shown to encode a t-SNARE protein (syntaxin), the molecular nature or Ror1 remained elusive. Ror1 was previously mapped to the centromeric region of the long arm of barley chromosome 1H. Here, we narrowed the barley Ror1 interval to 0.18 cM and initiated a chromosome walk using barley yeast artificial chromosome (YAC) clones, next-generation DNA sequencing and fluorescence in situ hybridization. Two non-overlapping YAC contigs containing Ror1 flanking genes were identified. Despite a high degree of synteny observed between barley and the sequenced genomes of the grasses rice (Oryza sativa), Brachypodium distachyon and Sorghum bicolor across the wider chromosomal area, the genes in the YAC contigs showed extensive interspecific rearrangements in orientation and order. Consequently, the position of a Ror1 homolog in these species could not be precisely predicted, nor was a barley gene co-segregating with Ror1 identified. These factors have prevented the molecular identification of the Ror1 gene for the time being.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Acevedo-Garcia, JohannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Collins, Nicholas C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ahmadinejad, NahalUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ma, LuUNSPECIFIEDorcid.org/0000-0001-5271-0686UNSPECIFIED
Houben, AndreasUNSPECIFIEDorcid.org/0000-0003-3419-239XUNSPECIFIED
Bednarek, PawelUNSPECIFIEDorcid.org/0000-0002-3064-7775UNSPECIFIED
Benjdia, MariamUNSPECIFIEDorcid.org/0000-0003-1554-5452UNSPECIFIED
Freialdenhoven, AndreasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmueller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nuernberg, PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Reinhardt, RichardUNSPECIFIEDorcid.org/0000-0001-9376-2132UNSPECIFIED
Schulze-Lefert, PaulUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Panstruga, RalphUNSPECIFIEDorcid.org/0000-0002-3756-8957UNSPECIFIED
URN: urn:nbn:de:hbz:38-471641
DOI: 10.1007/s00122-013-2186-6
Journal or Publication Title: Theor. Appl. Genet.
Volume: 126
Number: 12
Page Range: S. 2969 - 2983
Date: 2013
Publisher: SPRINGER
Place of Publication: NEW YORK
ISSN: 1432-2242
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HYBRIDIZATION SIGNAL-DETECTION; POWDERY MILDEW; DISEASE-RESISTANCE; GLUCOSINOLATE METABOLISM; CONFERRING RESISTANCE; GENE MOVEMENT; MLO GENE; GENOME; FUNGUS; RICEMultiple languages
Agronomy; Plant Sciences; Genetics & Heredity; HorticultureMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/47164

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