Universität zu Köln

Odenthal, M., Bollschweiler, E., Grimminger, P. P., Schroeder, W., Brabender, J., Drebber, U., Hoelscher, A. H., Metzger, R. and Vallboehmer, D. (2013). MicroRNA profiling in locally advanced esophageal cancer indicates a high potential of miR-192 in prediction of multimodality therapy response. Int. J. Cancer, 133 (10). S. 2454 - 2464. HOBOKEN: WILEY-BLACKWELL. ISSN 0020-7136

Full text not available from this repository.

Abstract

To identify possible predictive markers, our study aimed to characterize microRNA (miRNA) profiles of responder and nonresponder in the multimodality therapy of locally advanced esophageal cancer. Initially, a microarray-based approach was performed including eight patients with esophageal cancer. Patients received neoadjuvant chemoradiation followed by surgical resection. Major histopathological response was defined if resected specimens contained less than 10% vital tumor cells (major/minor response: 4/4 patients). Intratumoral RNA was isolated from both, pretherapeutic tissue biopsies in addition to corresponding surgical specimens. The profile of 768 miRNAs was analyzed in 16 specimens (preneoadjuvant and postneoadjuvant therapy). Selected miRNAs were than analyzed on pretherapeutic and post-therapeutic biopsies of 80 patients with esophageal cancer, who underwent multimodality therapy (major/minor response: 30/50 patients). Comprehensive miRNA profiling identified miRNAs in pretherapeutic biopsies that were significantly different between major/minor responders. Based on the microarray results, miR-192, miR-194 and miR-622 were selected and the dysregulated miRNAs were studied on an extended series of esophageal cancer patients. The expression of miR-192, miR-194 and miR-622 was significantly reduced after neoadjuvant therapy confirming the array profiling data. Importantly, the pretherapeutic intratumoral expression of miR-192 and miR-194 was significantly associated with the histopathologic response of esophageal squamous cell carcinoma to multimodal therapeutic treatment. Therefore, in patients with locally advanced esophageal cancer undergoing neoadjuvant chemoradiation followed by esophagectomy, miR-192 and miR-194 in pretherapeutic biopsies are considered as indicators of major histopathologic regression. What's new? Combining multiple modes of treatment is increasingly the standard of care in advanced esophageal cancer. However, while meta-analyses have indicated that patients with major histopathologic response may benefit from neoadjuvant regimens, predictive markers to determine patient response are missing. Here, the expression of a subset of microRNAs, identified from a panel of miRNAs in esophageal tumor specimens collected before and after neoadjuvant therapy, was found to be significantly reduced following treatment. Two markers in particular, miR-192 and miR-194, may be potential pre-therapeutic indicators of major histopathologic regression in esophageal cancer.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Odenthal, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bollschweiler, E. UNSPECIFIED UNSPECIFIED UNSPECIFIED Grimminger, P. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeder, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Brabender, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Drebber, U. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoelscher, A. H. UNSPECIFIED UNSPECIFIED UNSPECIFIED Metzger, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vallboehmer, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-471968
DOI:	10.1002/ijc.28253
Journal or Publication Title:	Int. J. Cancer
Volume:	133
Number:	10
Page Range:	S. 2454 - 2464
Date:	2013
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	0020-7136
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SQUAMOUS-CELL CARCINOMA; POSITRON-EMISSION-TOMOGRAPHY; NEOADJUVANT CHEMORADIOTHERAPY; SURVIVAL; EXPRESSION; ADENOCARCINOMA; PROLIFERATION; CHEMOTHERAPY; PROGNOSIS; RADIOCHEMOTHERAPY Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47196