Universität zu Köln

Manteniotis, Stavros, Lehmann, Ramona, Flegel, Caroline, Vogel, Felix, Hofreuter, Adrian, Schreiner, Benjamin S. P., Altmueller, Janine, Becker, Christian, Schoebel, Nicole, Hatt, Hanns and Gisselmann, Guenter (2013). Comprehensive RNA-Seq Expression Analysis of Sensory Ganglia with a Focus on Ion Channels and GPCRs in Trigeminal Ganglia. PLoS One, 8 (11). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

The specific functions of sensory systems depend on the tissue-specific expression of genes that code for molecular sensor proteins that are necessary for stimulus detection and membrane signaling. Using the Next Generation Sequencing technique (RNA-Seq), we analyzed the complete transcriptome of the trigeminal ganglia (TG) and dorsal root ganglia (DRG) of adult mice. Focusing on genes with an expression level higher than 1 FPKM (fragments per kilobase of transcript per million mapped reads), we detected the expression of 12984 genes in the TG and 13195 in the DRG. To analyze the specific gene expression patterns of the peripheral neuronal tissues, we compared their gene expression profiles with that of the liver, brain, olfactory epithelium, and skeletal muscle. The transcriptome data of the TG and DRG were scanned for virtually all known G-protein-coupled receptors (GPCRs) as well as for ion channels. The expression profile was ranked with regard to the level and specificity for the TG. In total, we detected 106 non-olfactory GPCRs and 33 ion channels that had not been previously described as expressed in the TG. To validate the RNA-Seq data, in situ hybridization experiments were performed for several of the newly detected transcripts. To identify differences in expression profiles between the sensory ganglia, the RNA-Seq data of the TG and DRG were compared. Among the differentially expressed genes (> 1 FPKM), 65 and 117 were expressed at least 10-fold higher in the TG and DRG, respectively. Our transcriptome analysis allows a comprehensive overview of all ion channels and G protein-coupled receptors that are expressed in trigeminal ganglia and provides additional approaches for the investigation of trigeminal sensing as well as for the physiological and pathophysiological mechanisms of pain.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Manteniotis, Stavros UNSPECIFIED UNSPECIFIED UNSPECIFIED Lehmann, Ramona UNSPECIFIED UNSPECIFIED UNSPECIFIED Flegel, Caroline UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogel, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Hofreuter, Adrian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schreiner, Benjamin S. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Altmueller, Janine UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoebel, Nicole UNSPECIFIED UNSPECIFIED UNSPECIFIED Hatt, Hanns UNSPECIFIED UNSPECIFIED UNSPECIFIED Gisselmann, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-472108
DOI:	10.1371/journal.pone.0079523
Journal or Publication Title:	PLoS One
Volume:	8
Number:	11
Date:	2013
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN-COUPLED RECEPTORS; DORSAL-ROOT GANGLION; ACTIVATED CHLORIDE CHANNEL; GATED CALCIUM-CHANNELS; POTENTIAL THERAPEUTIC TARGET; OF-FUNCTION MUTATIONS; SENSITIVE K+ CHANNEL; SODIUM-CHANNEL; POTASSIUM CHANNEL; MICE LACKING Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47210