Universität zu Köln

Tooming-Klunderud, Ave, Sogge, Hanne, Rounge, Trine Ballestad, Nederbragt, Alexander J., Lagesen, Karin ORCID: 0000-0003-1329-9032, Gloeckner, Gernot, Hayes, Paul K., Rohrlack, Thomas and Jakobsen, Kjetill S. (2013). From Green to Red: Horizontal Gene Transfer of the Phycoerythrin Gene Cluster between Planktothrix Strains. Appl. Environ. Microbiol., 79 (21). S. 6803 - 6813. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5336

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Abstract

Horizontal gene transfer is common in cyanobacteria, and transfer of large gene clusters may lead to acquisition of new functions and conceivably niche adaption. In the present study, we demonstrate that horizontal gene transfer between closely related Planktothrix strains can explain the production of the same oligopeptide isoforms by strains of different colors. Comparison of the genomes of eight Planktothrix strains revealed that strains producing the same oligopeptide isoforms are closely related, regardless of color. We have investigated genes involved in the synthesis of the photosynthetic pigments phycocyanin and phycoerythrin, which are responsible for green and red appearance, respectively. Sequence comparisons suggest the transfer of a functional phycoerythrin gene cluster generating a red phenotype in a strain that is otherwise more closely related to green strains. Our data show that the insertion of a DNA fragment containing the 19.7-kb phycoerythrin gene cluster has been facilitated by homologous recombination, also replacing a region of the phycocyanin operon. These findings demonstrate that large DNA fragments spanning entire functional gene clusters can be effectively transferred between closely related cyanobacterial strains and result in a changed phenotype. Further, the results shed new light on the discussion of the role of horizontal gene transfer in the sporadic distribution of large gene clusters in cyanobacteria, as well as the appearance of red and green strains.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Tooming-Klunderud, Ave UNSPECIFIED UNSPECIFIED UNSPECIFIED Sogge, Hanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Rounge, Trine Ballestad UNSPECIFIED UNSPECIFIED UNSPECIFIED Nederbragt, Alexander J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lagesen, Karin UNSPECIFIED orcid.org/0000-0003-1329-9032 UNSPECIFIED Gloeckner, Gernot UNSPECIFIED UNSPECIFIED UNSPECIFIED Hayes, Paul K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohrlack, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakobsen, Kjetill S. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-473266
DOI:	10.1128/AEM.01455-13
Journal or Publication Title:	Appl. Environ. Microbiol.
Volume:	79
Number:	21
Page Range:	S. 6803 - 6813
Date:	2013
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5336
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PEPTIDE SYNTHETASE GENE; CYANOBACTERIUM PLANKTOTHRIX; PHYLOGENETIC ANALYSES; MAXIMUM-LIKELIHOOD; EVOLUTION; DIVERSITY; MICROCYSTIN; ADAPTATION; DNA; PICOCYANOBACTERIA Multiple languages Biotechnology & Applied Microbiology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47326