Universität zu Köln

Niessen, Michaela T., Scott, Jeanie, Zielinski, Julia G., Vorhagen, Susanne, Sotiropoulou, Panagiota A., Blanpain, Cedric, Leitges, Michael ORCID: 0000-0003-4203-6995 and Niessen, Carien M. (2013). aPKC lambda controls epidermal homeostasis and stem cell fate through regulation of division orientation. J. Cell Biol., 202 (6). S. 887 - 901. NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 0021-9525

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Abstract

The atypical protein kinase C (aPKC) is a key regulator of polarity and cell fate in lower organisms. However, whether mammalian aPKCs control stem cells and fate in vivo is not known. Here we show that loss of aPKC lambda in a self-renewing epithelium, the epidermis, disturbed tissue homeostasis, differentiation, and stem cell dynamics, causing progressive changes in this tissue. This was accompanied by a gradual loss of quiescent hair follicle bulge stem cells and a temporary increase in proliferating progenitors. Lineage tracing analysis showed that loss of aPKC lambda altered the fate of lower bulge/hair germ stem cells. This ultimately led to loss of proliferative potential, stem cell exhaustion, alopecia, and premature aging. Inactivation of aPKC lambda produced more asymmetric divisions in different compartments, including the bulge. Thus, aPKC lambda is crucial for homeostasis of self-renewing stratifying epithelia, and for the regulation of cell fate, differentiation, and maintenance of epidermal bulge stem cells likely through its role in balancing symmetric and asymmetric division.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Niessen, Michaela T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Scott, Jeanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Zielinski, Julia G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vorhagen, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Sotiropoulou, Panagiota A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Blanpain, Cedric UNSPECIFIED UNSPECIFIED UNSPECIFIED Leitges, Michael UNSPECIFIED orcid.org/0000-0003-4203-6995 UNSPECIFIED Niessen, Carien M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-475880
DOI:	10.1083/jcb.201307001
Journal or Publication Title:	J. Cell Biol.
Volume:	202
Number:	6
Page Range:	S. 887 - 901
Date:	2013
Publisher:	ROCKEFELLER UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	0021-9525
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HAIR FOLLICLE; MOUSE EPIDERMIS; TARGETED DISRUPTION; SPINDLE ORIENTATION; PROGENITOR CELLS; SELF-RENEWAL; PROTEIN; DIFFERENTIATION; PROLIFERATION; POLARITY Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47588