Universität zu Köln

Engelstaedter, Verena, Heublein, Sabine, Schumacher, Anamur Lan, Lenhard, Miriam, Engelstaedter, Helen, Andergassen, Ulrich, Guenthner-Biller, Margit, Kuhn, Christina, Rack, Brigitte, Kupka, Markus, Mayr, Doris and Jeschke, Udo ORCID: 0000-0003-2623-3235 (2012). Mucin-1 and its relation to grade, stage and survival in ovarian carcinoma patients. BMC Cancer, 12. LONDON: BMC. ISSN 1471-2407

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Abstract

Background: Mucin-1 is known to be over-expressed by various human carcinomas and is shed into the circulation where it can be detected in patient's serum by specific anti-Mucin-1 antibodies, such as the tumour marker assays CA 15-3 and CA 27.29. The prognostic value of Mucin-1 expression in ovarian carcinoma remains uncertain. One aim of this study was to compare the concentrations of Mucin-1 in a cohort of patients with either benign or malignant ovarian tumours detected by CA 15-3 and CA 27.29. Another aim of this study was to evaluate Mucin-1 expression by immunohistochemistry in a different cohort of ovarian carcinoma patients with respect to grade, stage and survival. Methods: Patients diagnosed with and treated for ovarian tumours were included in the study. Patient characteristics, histology including histological subtype, tumour stage, grading and follow-up data were available from patient records. Serum Mucin-1 concentrations were measured with ELISA technology detecting CA 15-3 and CA 27.29, Mucin-1 tissue expression was determined by immunohistochemistry using the VU4H5 and VU3C6 anti-Mucin-1 antibodies. Statistical analysis was performed by using SPSS 18.0. Results: Serum samples of 118 patients with ovarian tumours were obtained to determine levels of Mucin-1. Median CA 15-3 and CA 27.29 concentrations were significantly higher in patients with malignant disease (p < 0.001) than in patients with benign disease. Paraffin-embedded tissue of 154 patients with ovarian carcinoma was available to determine Mucin-1 expression. The majority of patients presented with advanced stage disease at primary diagnosis. Median follow-up time was 11.39 years. Immunohistochemistry results for VU4H5 showed significant differences with respect to tumour grade, FIGO stage and overall survival. Patients with negative expression had a mean overall survival of 9.33 years compared to 6.27 years for patients with positive Mucin-1 expression. Conclusions: This study found significantly elevated Mucin-1 serum concentrations in ovarian carcinoma patients as compared to those women suffering from benign ovarian diseases. However, it needs to be noted that Mucin-1 concentrations in carcinoma patients showed a rather high variability. Results from immunohistochemistry indicate that Mucin-1 has a prognostic relevance in ovarian carcinomas when evaluating the expression by VU4H5 antibody.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Engelstaedter, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Heublein, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schumacher, Anamur Lan UNSPECIFIED UNSPECIFIED UNSPECIFIED Lenhard, Miriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Engelstaedter, Helen UNSPECIFIED UNSPECIFIED UNSPECIFIED Andergassen, Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Guenthner-Biller, Margit UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhn, Christina UNSPECIFIED UNSPECIFIED UNSPECIFIED Rack, Brigitte UNSPECIFIED UNSPECIFIED UNSPECIFIED Kupka, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Mayr, Doris UNSPECIFIED UNSPECIFIED UNSPECIFIED Jeschke, Udo UNSPECIFIED orcid.org/0000-0003-2623-3235 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476568
DOI:	10.1186/1471-2407-12-600
Journal or Publication Title:	BMC Cancer
Volume:	12
Date:	2012
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1471-2407
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MONOCLONAL-ANTIBODIES; BREAST-CANCER; MUC1; EXPRESSION; CHEMOTHERAPY; CA27.29; CA-15-3; CA15-3; ASSAYS; BR Multiple languages Oncology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47656