Universität zu Köln

Griese, Matthias, Heinrich, Stephanie, Ratjen, Felix ORCID: 0000-0003-4057-6592, Kabesch, Michael, Paul, Karl, Ballmann, Manfred, Rietschel, Ernst and Kappler, Matthias (2012). Surfactant Protein A in Cystic Fibrosis: Supratrimeric Structure and Pulmonary Outcome. PLoS One, 7 (12). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

Full text not available from this repository.

Abstract

Background: The state of oligomerization of surfactant associated protein-A (SP-A) monomers differs between individuals. This likely affects SP-A's functional properties and could thereby influence clinical status in patients with lung diseases. In this study we focus on SP-A structure in cystic fibrosis (CF) compared to both healthy subjects and disease controls. Methods: SP-A composition and function were assessed in both bronchoalveolar lavage (BAL) fluid and serum of 46 CF patients with mild disease, 25 patients with chronic bronchitis and 22 healthy subjects by gel chromatography and a functional agglutination assay. Relation of SP-A agglutination ability to disease severity of the subjects was explored. Results: SP-A was present in seven major oligomeric forms with the majority of SP-A being structurally organized as complex oligomeric forms. More complex oligomeric forms were associated with better SP-A function with regard to its agglutination ability. These forms were more frequently observed in BAL than in serum, but there were no differences between disease groups. In CF patients, more complex forms of SP-A were associated with better lung function. Conclusions: Organizational structure of SP-A affects its functional activity and is linked to disease severity in CF.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Griese, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinrich, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Ratjen, Felix UNSPECIFIED orcid.org/0000-0003-4057-6592 UNSPECIFIED Kabesch, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Paul, Karl UNSPECIFIED UNSPECIFIED UNSPECIFIED Ballmann, Manfred UNSPECIFIED UNSPECIFIED UNSPECIFIED Rietschel, Ernst UNSPECIFIED UNSPECIFIED UNSPECIFIED Kappler, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476843
DOI:	10.1371/journal.pone.0051050
Journal or Publication Title:	PLoS One
Volume:	7
Number:	12
Date:	2012
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language AIRWAY INFLAMMATION; PSEUDOMONAS-AERUGINOSA; YOUNG-CHILDREN; LUNG-FUNCTION; HOST-DEFENSE; DISEASE; OLIGOMERIZATION; PHAGOCYTOSIS; BINDING; SP-A1 Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47684