Universität zu Köln

Jaros, Julian A. J., Martins-de-Souza, Daniel ORCID: 0000-0003-3595-5846, Rahmoune, Hassan, Rothermundt, Matthias, Leweke, F. Markus ORCID: 0000-0002-8163-195X, Guest, Paul C. and Bahn, Sabine (2012). Protein phosphorylation patterns in serum from schizophrenia patients and healthy controls. J. Proteomics, 76. S. 43 - 56. AMSTERDAM: ELSEVIER. ISSN 1876-7737

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Abstract

Most proteomic studies to date have attempted to identify changes in protein levels without considering the effects of post-translational modifications (PTM). However, characteristic changes of PTM such as phosphorylation could be biologically informative, as these can give insights into disease and drug mechanisms of action at the functional level. With this in mind, we have conducted a comparative proteomic and phosphoproteomic analysis of blood sera from 20 antipsychotic-naive schizophrenia patients and 20 matched healthy controls. We used immobilised metal ion affinity chromatography (IMAC) for enrichment of phosphoproteins combined with label-free liquid chromatography-mass spectrometry (LC-MSE) for identification and measurement of protein and phosphoprotein levels. The LC-MSE analysis of both IMAC-fractions resulted in identification of 35 proteins with altered levels in schizophrenia. Analysis of the enriched fraction resulted in identification of 72 phosphoproteins with altered phosphorylation patterns. Of these, 59 showed changes in phosphorylation only, with no overall change in protein levels. This study provided evidence that schizophrenia patients feature serum abnormalities in phosphorylation of proteins involved in acute phase response and coagulation pathways. Further studies of such phosphorylation-specific changes could lead to a better understanding of the molecular aetiology of schizophrenia, and provide a means of biomarker identification for clinical studies. This article is part of a Special Issue entitled: Integrated omics. (C) 2012 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jaros, Julian A. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Martins-de-Souza, Daniel UNSPECIFIED orcid.org/0000-0003-3595-5846 UNSPECIFIED Rahmoune, Hassan UNSPECIFIED UNSPECIFIED UNSPECIFIED Rothermundt, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Leweke, F. Markus UNSPECIFIED orcid.org/0000-0002-8163-195X UNSPECIFIED Guest, Paul C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bahn, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-476902
DOI:	10.1016/j.jprot.2012.05.027
Journal or Publication Title:	J. Proteomics
Volume:	76
Page Range:	S. 43 - 56
Date:	2012
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1876-7737
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEOMIC ANALYSIS; APOLIPOPROTEIN A1; COMPLEMENT; MARKERS; BRAIN; CLUSTERIN; IDENTIFICATION; DISCOVERY; BIOMARKER; SYSTEM Multiple languages Biochemical Research Methods Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47690