Universität zu Köln

Watzek, Nico, Scherbl, Denise, Feld, Julia, Berger, Franz, Doroshyenko, Oxana, Fuhr, Uwe, Tomalik-Scharte, Dorota, Baum, Matthias, Eisenbrand, Gerhard and Richling, Elke (2012). Profiling of mercapturic acids of acrolein and acrylamide in human urine after consumption of potato crisps. Mol. Nutr. Food Res., 56 (12). S. 1825 - 1838. HOBOKEN: WILEY-BLACKWELL. ISSN 1613-4125

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Abstract

Scope Acrolein (AC) and acrylamide (AA) are food contaminants generated by heat treatment. We studied human exposure after consumption of potato crisps by monitoring excretion of mercapturic acids (MAs) in urine. Methods and results MA excretion was monitored in human urine collected up to 72 h after ingestion of a test meal of experimental (study 1: 1 mg AA/150 g) or commercially available (study 2: 44 mu g AA plus 4.6 mu g AC/175 g) potato crisps. MA contents were analysed after purification via SPE using HPLC-ESI-MS/MS. On the basis of the area under the curve values of MAs excreted in urine, the total excretion of AC-related MAs exceeded that of AA-related MAs up to 12 times in study 1 and up to four times in study 2. Remarkably, AC content of potato crisps of study 2 was found to be only about 1/10 the AA content, as determined by isotope dilution headspace GC/MS. Conclusion Our results indicate substantially higher exposure to AC from potato crisps than to AA. Total AC in such foods may encompass bioavailable AC forms not detected by headspace GC/MS. Both findings may also apply to other heat processed foods.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Watzek, Nico UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherbl, Denise UNSPECIFIED UNSPECIFIED UNSPECIFIED Feld, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Berger, Franz UNSPECIFIED UNSPECIFIED UNSPECIFIED Doroshyenko, Oxana UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuhr, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Tomalik-Scharte, Dorota UNSPECIFIED UNSPECIFIED UNSPECIFIED Baum, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Eisenbrand, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Richling, Elke UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-477538
DOI:	10.1002/mnfr.201200323
Journal or Publication Title:	Mol. Nutr. Food Res.
Volume:	56
Number:	12
Page Range:	S. 1825 - 1838
Date:	2012
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1613-4125
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TANDEM MASS-SPECTROMETRY; DNA ADDUCT FORMATION; RISK-ASSESSMENT; LIQUID-CHROMATOGRAPHY; CYTOCHROME-P450 2E1; FATTY-ACIDS; HUMAN BLOOD; HUMAN LUNG; IN-VIVO; GLYCIDAMIDE Multiple languages Food Science & Technology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47753