Universität zu Köln

Choorapoikayil, Suma, Schoepe, Jan, Buchinger, Sebastian ORCID: 0000-0003-4859-8972 and Schomburg, Dietmar ORCID: 0000-0002-3354-822X (2012). Analysis of in vivo Function of Predicted Isoenzymes-A Metabolomic Approach. OMICS, 16 (12). S. 668 - 681. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-8100

Full text not available from this repository.

Abstract

Isoenzymes occur in all organisms. Often, they are regulated with respect to environmental perturbations to assure metabolic flexibility. In bioinformatics-driven functional genome annotations, the presence of isoenzymes is frequently predicted. It is desirable to verify the functions of putative isoenzymes experimentally for a correct estimation of the metabolic capacities in a given organism. Using metabolome analysis, we investigated two knockout mutants of putative shikimate dehydrogenases (SDH) in Corynebacterium glutamicum (C. glutamicum). Here, we show that different metabolic responses to defined gene deactivations of the putative SDHs clearly indicate different functions of these enzymes. Our investigation revealed that the gene product of open reading frame Cg1835 is the essential SDH in C. glutamicum, whereas it is more likely that the gene product of the open reading frame Cg1283 belongs to the SDH-like enzyme family (SDH-L). The results of the metabolome analysis are verified with two independent methods. First, by in vitro characterization of kinetic constants after heterologous expression, and second, by measurement of SDH activity in raw cell extracts.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Choorapoikayil, Suma UNSPECIFIED UNSPECIFIED UNSPECIFIED Schoepe, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Buchinger, Sebastian UNSPECIFIED orcid.org/0000-0003-4859-8972 UNSPECIFIED Schomburg, Dietmar UNSPECIFIED orcid.org/0000-0002-3354-822X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-478179
DOI:	10.1089/omi.2012.0055
Journal or Publication Title:	OMICS
Volume:	16
Number:	12
Page Range:	S. 668 - 681
Date:	2012
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1557-8100
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GAS CHROMATOGRAPHY/MASS SPECTROMETRY; SHIKIMATE DEHYDROGENASE AROE; L-LYSINE PRODUCTION; CORYNEBACTERIUM-GLUTAMICUM; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; SACCHAROMYCES-CEREVISIAE; MULTIORGANISM DATABASE; MOLECULAR-CLONING; GENE DELETION Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47817