De Kock, Joery ORCID: 0000-0002-4078-4896, Najar, Mehdi, Bolleyn, Jennifer, Al Battah, Feras, Rodrigues, Robim M., Buyl, Karolien ORCID: 0000-0002-4889-9284, Raicevic, Gordana, Govaere, Olivier ORCID: 0000-0002-4426-6930, Branson, Steven, Meganathan, Kesavan, Gaspar, John Antonydas, Roskams, Tania ORCID: 0000-0002-2816-1530, Sachinidis, Agapios, Lagneaux, Laurence, Vanhaecke, Tamara ORCID: 0000-0002-6685-7299 and Rogiers, Vera ORCID: 0000-0003-0635-7740 (2012). Mesoderm-Derived Stem Cells: The Link Between the Transcriptome and Their Differentiation Potential. Stem Cells Dev., 21 (18). S. 3309 - 3324. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-8534

Full text not available from this repository.

Abstract

Human adult stem cells (hASCs) have become an attractive source for autologous cell transplantation, tissue engineering, developmental biology, and the generation of human-based alternative in vitro models. Among the 3 germ cell layers, the mesoderm is the origin of today's most widely used and characterized hASC populations. A variety of isolated nonhematopoietic mesoderm-derived stem cell populations exist, and all of them show important differences in terms of function, efficacy, and differentiation potential both in vivo and in vitro. To better understand whether the intrinsic properties of these cells contribute to the overall differentiation potential of hASCs, we compared the global gene expression profiles of 4 mesoderm-derived stem cell populations: human adipose tissue-derived stromal cells, human bone marrow-derived stromal cells (hBMSCs), human (fore) skin-derived precursor cells (hSKPs), and human Wharton's jelly-derived mesenchymal stem cells (hWJs). Significant differences in gene expression profiles were detected between distinct stem cell types. hSKPs predominantly expressed genes involved in neurogenesis, skin, and bone development, whereas hWJs and, to some extent, hBMSCs showed an increased expression of genes involved in cardiovascular and liver development. Interestingly, the observed differential gene expression of distinct hASCs could be linked to existing differentiation data in which hASCs were differentiated toward specific cell types. As such, our data suggest that the intrinsic gene expression of the undifferentiated stem cells has an important impact on their overall differentiation potential as well as their application in stem cell-based research. Yet, the factors that define these intrinsic properties remain to be determined.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
De Kock, JoeryUNSPECIFIEDorcid.org/0000-0002-4078-4896UNSPECIFIED
Najar, MehdiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bolleyn, JenniferUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Al Battah, FerasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rodrigues, Robim M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Buyl, KarolienUNSPECIFIEDorcid.org/0000-0002-4889-9284UNSPECIFIED
Raicevic, GordanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Govaere, OlivierUNSPECIFIEDorcid.org/0000-0002-4426-6930UNSPECIFIED
Branson, StevenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Meganathan, KesavanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gaspar, John AntonydasUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roskams, TaniaUNSPECIFIEDorcid.org/0000-0002-2816-1530UNSPECIFIED
Sachinidis, AgapiosUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lagneaux, LaurenceUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vanhaecke, TamaraUNSPECIFIEDorcid.org/0000-0002-6685-7299UNSPECIFIED
Rogiers, VeraUNSPECIFIEDorcid.org/0000-0003-0635-7740UNSPECIFIED
URN: urn:nbn:de:hbz:38-478218
DOI: 10.1089/scd.2011.0723
Journal or Publication Title: Stem Cells Dev.
Volume: 21
Number: 18
Page Range: S. 3309 - 3324
Date: 2012
Publisher: MARY ANN LIEBERT, INC
Place of Publication: NEW ROCHELLE
ISSN: 1557-8534
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
MESENCHYMAL STROMAL CELLS; HUMAN BONE-MARROW; MYELINATING SCHWANN-CELLS; HUMAN UMBILICAL-CORD; TISSUE IN-VITRO; ADIPOSE-TISSUE; HEPATOCYTE DIFFERENTIATION; HEPATIC DIFFERENTIATION; MULTIPOTENT CHARACTER; ADHESION MOLECULESMultiple languages
Cell & Tissue Engineering; Hematology; Medicine, Research & Experimental; TransplantationMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/47821

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item