Universität zu Köln

Schulz, Julia C., Stumpf, Patrick S., Katsen-Globa, Alisa, Sachinidis, Agapios, Hescheler, Juergen and Zimmermann, Heiko ORCID: 0000-0001-8595-1304 (2012). First steps towards the successful surface-based cultivation of human embryonic stem cells in hanging drop systems. Eng. Life Sci., 12 (6). S. 584 - 588. HOBOKEN: WILEY-BLACKWELL. ISSN 1618-0240

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Abstract

Miniaturization and parallelization of cell culture procedures are in focus of research in order to develop test platforms with low material consumption and increased standardization for toxicity and drug screenings. The cultivation in hanging drops (HDs) is a convenient and versatile tool for biological applications and represents an interesting model system for the screening applications due to its uniform shape, the advantageous gas supply, and the small volume. However, its application has so far been limited to non#x02010;adherent and aggregate forming cells. Here, we describe for the first time the proof-of-principle regarding the adherent cultivation of human embryonic stem cells in HD. For this microcarriers were added to the droplet as dynamic cultivation surfaces resulting in a maintained pluripotency and proliferation capacity for 10 days. This enables the HD technique to be extended to the cultivation of adherence-dependent stem cells. Also, the possible automation of this method by implementation of liquid handling systems opens new possibilities for miniaturized screenings, the improvement of cultivation and differentiation conditions, and toxicity and drug development.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schulz, Julia C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stumpf, Patrick S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Katsen-Globa, Alisa UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, Agapios UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Zimmermann, Heiko UNSPECIFIED orcid.org/0000-0001-8595-1304 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-479416
DOI:	10.1002/elsc.201100213
Journal or Publication Title:	Eng. Life Sci.
Volume:	12
Number:	6
Page Range:	S. 584 - 588
Date:	2012
Publisher:	WILEY-BLACKWELL
Place of Publication:	HOBOKEN
ISSN:	1618-0240
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO; HUMAN BLASTOCYSTS; DIFFERENTIATION; CULTURES; LINES Multiple languages Biotechnology & Applied Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47941