Universität zu Köln

Zach, Frank ORCID: 0000-0003-1168-8106, Grassmann, Felix ORCID: 0000-0003-1390-7528, Langmann, Thomas, Sorusch, Nasrin, Wolfrum, Uwe and Stoehr, Heidi (2012). The retinitis pigmentosa 28 protein FAM161A is a novel ciliary protein involved in intermolecular protein interaction and microtubule association. Hum. Mol. Genet., 21 (21). S. 4573 - 4587. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2083

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Abstract

Loss-of-function mutations in the gene encoding FAM161A were recently discovered as the cause for RP28, an autosomal recessive form of retinitis pigmentosa. To initiate the characterization of the cellular role of FAM161A in the retina, we focused on its subcellular localization and conducted in vitro studies to identify FAM161A-interacting proteins and associated cellular structures. Immunohistochemistry revealed the presence of mouse FAM161A in the photoreceptor inner segments, the synaptic regions of the outer and inner plexiform layers and the ganglion cells. In mouse and human retinal sections from unfixed eyes, FAM161A localized to the ciliary region linking photoreceptor outer and inner segments. High-resolution immunofluorescence and immunoelectron microscopy mapped FAM161A to the connecting cilium, the basal body region and the adjacent centriole. Ectopic FAM161A was found in the centrosome and concentrated at the base of primary cilia in cultured cells. In addition, overexpressed FAM161A was clearly associated with microtubules during interphase and mitosis. The presence of FAM161A increased microtubule acetylation and stabilization. We further show that the evolutionarily conserved UPF0564 domain of FAM161A is crucial for its binding to microtubules and mediates homo- and heterotypic FAM161A and FAM161B interaction. In conclusion, our study shows that FAM161A is a microtubule-associated ciliary protein presumably involved in microtubule stabilization to maintain the microtubule tracks and/or in transport processes along microtubules in photoreceptors and other retinal cell types.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zach, Frank UNSPECIFIED orcid.org/0000-0003-1168-8106 UNSPECIFIED Grassmann, Felix UNSPECIFIED orcid.org/0000-0003-1390-7528 UNSPECIFIED Langmann, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Sorusch, Nasrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolfrum, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Stoehr, Heidi UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-479433
DOI:	10.1093/hmg/dds268
Journal or Publication Title:	Hum. Mol. Genet.
Volume:	21
Number:	21
Page Range:	S. 4573 - 4587
Date:	2012
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2083
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTRAFLAGELLAR TRANSPORT; PHOTORECEPTOR CELLS; TUBULIN ACETYLATION; CONNECTING CILIUM; ALPHA-TUBULIN; VESICLE TRANSPORT; TRANSITION ZONE; LOCALIZATION; BINDING; CILIOGENESIS Multiple languages Biochemistry & Molecular Biology; Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47943