Universität zu Köln

Hoerster, Robert, Muether, Philipp S., Vierkotten, Sarah, Schroeder, Susanne, Kirchhof, Bernd and Fauser, Sascha (2012). In-vivo and ex-vivo characterization of laser-induced choroidal neovascularization variability in mice. Graefes Arch. Clin. Exp. Ophthalmol., 250 (11). S. 1579 - 1587. NEW YORK: SPRINGER. ISSN 0721-832X

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Abstract

Background Retinal argon laser coagulation is an established procedure for induction of choroidal neovascularization (CNV) in rodents. This study aimed to evaluate the in-vivo and ex-vivo morphology and variability of laser-induced CNV spots over time. Methods Female C57/Bl/6 mice, 3-6 months of age, were treated with five spots of retinal argon laser coagulation per eye (150 mW, 100 ms, 50 mu m). In-vivo fluorescein angiography (FA) and standard high-resolution spectral-domain optical coherence tomography (SD-OCT) were performed on day (d) 0, d1, d4, d7, d14 and d21. Ex-vivo histology, CD31 immunostaining, flatmount and confocal microscopy were also conducted. CNV size in the retinal and choroidal focus, CNV morphology, central retinal thickness (CRT) and FA CNV activity grading were assessed in-vivo at all times and compared to the ex-vivo assessments. Results SD-OCT revealed sub-retinal and intra-retinal fluid, and permitted evaluation of longitudinal morphologic changes of the induced CNV. Laser spot area in FA and CRT in SD-OCT did not differ in longitudinal evaluation. CNV could not be consistently outlined on SD-OCT images, and CNV volume as assessed on SD-OCT did not change over time. Significant CNV activity changes were only found in FA CNV activity grading, peaking on d4 and decreasing by d7. Conclusions Non-invasive SD-OCT provides additional morphological information on laser-induced CNV. However, reliable evaluation of CNV requires FA. Spontaneous regression of CNV activity within 14 days after induction has to be taken into account when utilizing this model for testing the efficacies of potential future treatments.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hoerster, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED Muether, Philipp S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vierkotten, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Schroeder, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirchhof, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Fauser, Sascha UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-479876
DOI:	10.1007/s00417-012-1990-z
Journal or Publication Title:	Graefes Arch. Clin. Exp. Ophthalmol.
Volume:	250
Number:	11
Page Range:	S. 1579 - 1587
Date:	2012
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	0721-832X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OPTICAL COHERENCE TOMOGRAPHY; MACULAR DEGENERATION; MACULOPATHY; PREVALENCE Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/47987