Universität zu Köln

Walpurgis, Katja, Kohler, Maxie, Thomas, Andreas ORCID: 0000-0003-1199-0743, Wenzel, Folker, Geyer, Hans, Schaenzer, Wilhelm and Thevis, Mario (2012). Storage-induced changes of the cytosolic red blood cell proteome analyzed by 2D DIGE and high-resolution/high-accuracy MS. Proteomics, 12 (21). S. 3263 - 3273. HOBOKEN: WILEY. ISSN 1615-9861

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Abstract

The storage of packed red blood cells (RBCs) is associated with the development of morphological and biochemical changes leading to a reduced posttransfusion functionality and viability of the cells. Within this study, 2D DIGE and high-resolution/high-accuracy Orbitrap MS were used to analyze the storage-induced changes of the cytosolic RBC proteome and identify characteristic protein patterns and potential marker proteins for the assessment of RBC storage lesions. Leukodepleted RBC concentrates were stored according to standard blood bank conditions for 0, 7, 14, 28, and 42 days and analyzed by using a characterized and validated protocol. Following statistical evaluation, a total of 14 protein spots were found to be significantly altered after 42 days of ex vivo storage. Protein identification was accomplished by tryptic digestion and LC-MS/MS and three proteins potentially useful as biomarkers for RBC aging comprising transglutaminase 2, beta actin, and copper chaperone for superoxide dismutase were selected and validated by western blot analysis. These can serve as a basis for the development of a screening assay to detect RBC storage lesions and autologous blood doping in sports.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Walpurgis, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Kohler, Maxie UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Wenzel, Folker UNSPECIFIED UNSPECIFIED UNSPECIFIED Geyer, Hans UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-480708
DOI:	10.1002/pmic.201200280
Journal or Publication Title:	Proteomics
Volume:	12
Number:	21
Page Range:	S. 3263 - 3273
Date:	2012
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1615-9861
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MASS-SPECTROMETRY; TRANSFUSION MEDICINE; SUPEROXIDE-DISMUTASE; ERYTHROCYTE PROTEINS; HEMOGLOBIN-DEPLETION; GEL-ELECTROPHORESIS; MEMBRANE-PROTEINS; CROSS-LINKING; ORBITRAP MS; TRANSGLUTAMINASE Multiple languages Biochemical Research Methods; Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48070