Universität zu Köln

Massoudi, Dawiyat, Malecaze, Francois, Soler, Vincent, Butterworth, Jacqueline, Erraud, Angelique, Fournie, Pierre, Koch, Manuel ORCID: 0000-0002-2962-7814 and Galiacy, Stephane D. (2012). NC1 Long and NC3 Short Splice Variants of Type XII Collagen Are Overexpressed during Corneal Scarring. Invest. Ophthalmol. Vis. Sci., 53 (11). S. 7246 - 7257. ROCKVILLE: ASSOC RESEARCH VISION OPHTHALMOLOGY INC. ISSN 1552-5783

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Abstract

PURPOSE. To investigate type XII collagen expression in corneal scars in vivo. METHODS. Type XII collagen protein expression was evaluated by immunohistochemistry in human corneal scars and in a mouse model of corneal scarring at several time points (from day 7 to day 210) after full-thickness excision. Alternative splice variants of the NC3 and NC1 domains of type XII collagen were investigated in the mouse wound-healing model using RT-PCR. RESULTS. Type XII collagen was overexpressed in human corneal scars in areas that were also positive for alpha-smooth muscle actin staining. In a mouse model of corneal wound injury we found that at 14 and 21 days postexcision, type XII collagen was largely concentrated in the subepithelial region of the cornea, especially in and near the wound bed. By 28 days postexcision, expression of type XII collagen decreased but remained higher than that in controls. NC3 short form is the main form expressed in the cornea during the wound-healing process. After injury, the NC1 long splice variant mRNA was the most highly overexpressed variant in the cornea, especially in the epithelium (X2.7, 3.72, and 5.57 at days 7, 14, and 21, respectively, P < 0.01 to 0.001 compared with uninjured samples). Corneal scars from a 7-month-old mouse revealed an overexpression of type XII collagen in the wound area similar to what we observed in human corneal scars. CONCLUSIONS. Type XII collagen is overexpressed in permanent human and mouse corneal scars and could represent a new target to treat corneal scarring. (Invest Ophthalmol Vis Sci. 2012; 53: 7246-7256) DOI: 10.1167/iovs.11-8592

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Massoudi, Dawiyat UNSPECIFIED UNSPECIFIED UNSPECIFIED Malecaze, Francois UNSPECIFIED UNSPECIFIED UNSPECIFIED Soler, Vincent UNSPECIFIED UNSPECIFIED UNSPECIFIED Butterworth, Jacqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Erraud, Angelique UNSPECIFIED UNSPECIFIED UNSPECIFIED Fournie, Pierre UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Galiacy, Stephane D. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481316
DOI:	10.1167/iovs.11-8592
Journal or Publication Title:	Invest. Ophthalmol. Vis. Sci.
Volume:	53
Number:	11
Page Range:	S. 7246 - 7257
Date:	2012
Publisher:	ASSOC RESEARCH VISION OPHTHALMOLOGY INC
Place of Publication:	ROCKVILLE
ISSN:	1552-5783
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TISSUE-SPECIFIC EXPRESSION; PHOTOREFRACTIVE KERATECTOMY; MESSENGER-RNA; HAZE; FIBRILLOGENESIS; KERATOCONUS; REGRESSION; FIBROSIS; REGIONS; DECORIN Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48131