Universität zu Köln

Hartwig, Benjamin, James, Geo Velikkakam, Konrad, Kathryn, Schneeberger, Korbinian and Turck, Franziska ORCID: 0000-0002-4982-5949 (2012). Fast Isogenic Mapping-by-Sequencing of Ethyl Methanesulfonate-Induced Mutant Bulks. Plant Physiol., 160 (2). S. 591 - 601. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-2548

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Abstract

Mapping-by-sequencing (or SHOREmapping) has revitalized the powerful concept of forward genetic screens in plants. However, as in conventional genetic mapping approaches, mapping-by-sequencing requires phenotyping of mapping populations established from crosses between two diverged accessions. In addition to the segregation of the focal phenotype, this introduces natural phenotypic variation, which can interfere with the recognition of quantitative phenotypes. Here, we demonstrate how mapping-by-sequencing and candidate gene identification can be performed within the same genetic background using only mutagen-induced changes as segregating markers. Using a previously unknown suppressor of mutants of like heterochromatin protein1 (lhp1), which in its functional form is involved in chromatin-mediated gene repression, we identified three closely linked ethyl methanesulfonate-induced changes as putative candidates. In order to assess allele frequency differences between such closely linked mutations, we introduced deep candidate resequencing using the new Ion Torrent Personal Genome Machine sequencing platform to our mutant identification pipeline and thereby reduced the number of causal candidate mutations to only one. Genetic analysis of two independent additional alleles confirmed that this mutation was causal for the suppression of lhp1.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hartwig, Benjamin UNSPECIFIED UNSPECIFIED UNSPECIFIED James, Geo Velikkakam UNSPECIFIED UNSPECIFIED UNSPECIFIED Konrad, Kathryn UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneeberger, Korbinian UNSPECIFIED UNSPECIFIED UNSPECIFIED Turck, Franziska UNSPECIFIED orcid.org/0000-0002-4982-5949 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481570
DOI:	10.1104/pp.112.200311
Journal or Publication Title:	Plant Physiol.
Volume:	160
Number:	2
Page Range:	S. 591 - 601
Date:	2012
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-2548
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FLOWERING-LOCUS-T; HETEROCHROMATIN PROTEIN-1; GROUP GENES; ARABIDOPSIS; POLYCOMB; DROSOPHILA; HOMOLOG; SCREEN; IDENTIFICATION; TRANSPOSASES Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48157