Universität zu Köln

Hess, Cornelius, Thomas, Andreas ORCID: 0000-0003-1199-0743, Thevis, Mario, Stratmann, Bernd, Quester, Wulf, Tschoepe, Diethelm, Madea, Burkhard and Musshoff, Frank (2012). Simultaneous determination and validated quantification of human insulin and its synthetic analogues in human blood serum by immunoaffinity purification and liquid chromatography-mass spectrometry. Anal. Bioanal. Chem., 404 (6-7). S. 1813 - 1823. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1618-2642

Full text not available from this repository.

Abstract

Possible fatal complications of human insulin and its synthetic analogues like hypoglycemia require precise classification and quantitative determination of these drugs both for clinical purposes as well as for forensic toxicologists. A procedure was developed for the identification and quantification of human insulin and different long-acting as well as short-acting synthetic insulins in human blood serum specimens. After an immunoaffinity purification step and separation by liquid chromatography, the insulins were characterized by their five- or sixfold protonated molecule ions and diagnostic product ions. Clinical samples of 207 diabetic and 50 non-diabetic patients after the administration of human insulin or oral antidiabetics and forensic samples were analyzed for human/synthetic insulin concentrations. The method was validated according to international guidelines. Limits of detection of the insulins ranged between 1.3 and 2.8 mu U/ml. Recoveries ranged between 33.2 % and 51.7 %. Precision data was in accordance with international guidelines. Clinical samples showed concentrations of human insulin lower than 301 mu U/ml. Our liquid chromatography tandem mass spectrometry procedure allows unambiguous identification and quantification of the intact human insulin and its intact synthetic analogues HumalogA (R), NovologA (R), ApidraA (R), LantusA (R), and LevemirA (R) in human blood serum in clinical and overdose cases. The assay could be successfully tested in patients with diabetes mellitus on therapy with insulins or oral antidiabetics.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hess, Cornelius UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Stratmann, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Quester, Wulf UNSPECIFIED UNSPECIFIED UNSPECIFIED Tschoepe, Diethelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Madea, Burkhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Musshoff, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481689
DOI:	10.1007/s00216-012-6271-5
Journal or Publication Title:	Anal. Bioanal. Chem.
Volume:	404
Number:	6-7
Page Range:	S. 1813 - 1823
Date:	2012
Publisher:	SPRINGER HEIDELBERG
Place of Publication:	HEIDELBERG
ISSN:	1618-2642
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DOPING CONTROL PURPOSES; AMERICAN ASSOCIATION; SEVERE HYPOGLYCEMIA; DEGRADING ENZYME; HUMAN PLASMA; HUMAN URINE; PROINSULIN; IMMUNOASSAY; DEGRADATION; PRODUCTS Multiple languages Biochemical Research Methods; Chemistry, Analytical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48168