Universität zu Köln

Gigolashvili, Tamara ORCID: 0000-0002-0416-4796, Geier, Melanie, Ashykhmina, Natallia, Frerigmann, Henning ORCID: 0000-0002-7067-2721, Wulfert, Sabine, Krueger, Stephan, Mugford, Sarah G., Kopriva, Stanislav ORCID: 0000-0002-7416-6551, Haferkamp, Ilka and Fluegge, Ulf-Ingo (2012). The Arabidopsis Thylakoid ADP/ATP Carrier TAAC Has an Additional Role in Supplying Plastidic Phosphoadenosine 5 '-Phosphosulfate to the Cytosol. Plant Cell, 24 (10). S. 4187 - 4205. ROCKVILLE: AMER SOC PLANT BIOLOGISTS. ISSN 1532-298X

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Abstract

3'-Phosphoadenosine 5'-phosphosulfate (PAPS) is the high-energy sulfate donor for sulfation reactions. Plants produce some PAPS in the cytosol, but it is predominantly produced in plastids. Accordingly, PAPS has to be provided by plastids to serve as a substrate for sulfotransferase reactions in the cytosol and the Golgi apparatus. We present several lines of evidence that the recently described Arabidopsis thaliana thylakoid ADP/ATP carrier TAAC transports PAPS across the plastid envelope and thus fulfills an additional function of high physiological relevance. Transport studies using the recombinant protein revealed that it favors PAPS, 3'-phosphoadenosine 5'-phosphate, and ATP as substrates; thus, we named it PAPST1. The protein could be detected both in the plastid envelope membrane and in thylakoids, and it is present in plastids of autotrophic and heterotrophic tissues. TAAC/PAPST1 belongs to the mitochondrial carrier family in contrast with the known animal PAPS transporters, which are members of the nucleotide- sugar transporter family. The expression of the PAPST1 gene is regulated by the same MYB transcription factors also regulating the biosynthesis of sulfated secondary metabolites, glucosinolates. Molecular and physiological analyses of papst1 mutant plants indicate that PAPST1 is involved in several aspects of sulfur metabolism, including the biosynthesis of thiols, glucosinolates, and phytosulfokines.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gigolashvili, Tamara UNSPECIFIED orcid.org/0000-0002-0416-4796 UNSPECIFIED Geier, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Ashykhmina, Natallia UNSPECIFIED UNSPECIFIED UNSPECIFIED Frerigmann, Henning UNSPECIFIED orcid.org/0000-0002-7067-2721 UNSPECIFIED Wulfert, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Krueger, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Mugford, Sarah G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Haferkamp, Ilka UNSPECIFIED UNSPECIFIED UNSPECIFIED Fluegge, Ulf-Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-482593
DOI:	10.1105/tpc.112.101964
Journal or Publication Title:	Plant Cell
Volume:	24
Number:	10
Page Range:	S. 4187 - 4205
Date:	2012
Publisher:	AMER SOC PLANT BIOLOGISTS
Place of Publication:	ROCKVILLE
ISSN:	1532-298X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADENINE-NUCLEOTIDE TRANSPORTER; GLUCOSINOLATE BIOSYNTHESIS; PROTEIN IMPORT; TYROSYLPROTEIN SULFOTRANSFERASE; TRANSCRIPTION FACTORS; TRANSIENT EXPRESSION; MOLECULAR-CLONING; ATP/ADP CARRIER; IDENTIFICATION; PATHWAY Multiple languages Biochemistry & Molecular Biology; Plant Sciences; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48259