Universität zu Köln

Parajuli, Laxmi Kumar, Nakajima, Chikako ORCID: 0000-0003-3071-8585, Kulik, Akos, Matsui, Ko ORCID: 0000-0003-1068-9705, Schneider, Toni, Shigemoto, Ryuichi ORCID: 0000-0001-8761-9444 and Fukazawa, Yugo ORCID: 0000-0001-7436-8797 (2012). Quantitative Regional and Ultrastructural Localization of the Ca(v)2.3 Subunit of R-type Calcium Channel in Mouse Brain. J. Neurosci., 32 (39). S. 13555 - 13568. WASHINGTON: SOC NEUROSCIENCE. ISSN 0270-6474

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Abstract

R-type calcium channels (RTCCs) are well known for their role in synaptic plasticity, but little is known about their subcellular distribution across various neuronal compartments. Using subtype-specific antibodies, we characterized the regional and subcellular localization of Ca(v)2.3 in mice and rats at both light and electron microscopic levels. Ca(v)2.3 immunogold particles were found to be predominantly presynaptic in the interpeduncular nucleus, but postsynaptic in other brain regions. Serial section analysis of electron microscopic images from the hippocampal CA1 revealed a higher density of immunogold particles in the dendritic shaft plasma membrane compared with the pyramidal cell somata. However, the labeling densities were not significantly different among the apical, oblique, or basal dendrites. Immunogold particles were also observed over the plasma membrane of dendritic spines, including both synaptic and extra-synaptic sites. Individual spine heads contained <20 immunogold particles, with an average density of similar to 260 immunoparticles per mu m(3) spine head volume, in accordance with the density of RTCCs estimated using calcium imaging (Sabatini and Svoboda, 2000). The Ca(v)2.3 density was variable among similar-sized spine heads and did not correlate with the density in the parent dendrite, implying that spines are individual calcium compartments operating autonomously from their parent dendrites.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Parajuli, Laxmi Kumar UNSPECIFIED UNSPECIFIED UNSPECIFIED Nakajima, Chikako UNSPECIFIED orcid.org/0000-0003-3071-8585 UNSPECIFIED Kulik, Akos UNSPECIFIED UNSPECIFIED UNSPECIFIED Matsui, Ko UNSPECIFIED orcid.org/0000-0003-1068-9705 UNSPECIFIED Schneider, Toni UNSPECIFIED UNSPECIFIED UNSPECIFIED Shigemoto, Ryuichi UNSPECIFIED orcid.org/0000-0001-8761-9444 UNSPECIFIED Fukazawa, Yugo UNSPECIFIED orcid.org/0000-0001-7436-8797 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-482774
DOI:	10.1523/JNEUROSCI.1142-12.2012
Journal or Publication Title:	J. Neurosci.
Volume:	32
Number:	39
Page Range:	S. 13555 - 13568
Date:	2012
Publisher:	SOC NEUROSCIENCE
Place of Publication:	WASHINGTON
ISSN:	0270-6474
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DENDRITIC SPINES; SUBCELLULAR-DISTRIBUTION; PYRAMIDAL CELLS; HIPPOCAMPAL; EXPRESSION; PLASTICITY; RELEASE; RECEPTORS; GLUTAMATE; SIGNALS Multiple languages Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48277