Universität zu Köln

Aydiner, Aylin, Luesebrink, Jessica, Schildgen, Verena, Winterfeld, Ingo, Knuever, Oliver, Schwarz, Katja, Messler, Sabine, Schildgen, Oliver ORCID: 0000-0003-4297-9627 and Mattner, Frauke (2012). Comparison of Two Commercial PCR Methods for Methicillin-Resistant Staphylococcus aureus (MRSA) Screening in a Tertiary Care Hospital. PLoS One, 7 (9). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

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Abstract

Nose/throat-swabs from 1049 patients were screened for MRSA using CHROMagar MRSA, LightCycler Advanced MRSA, and Detect-Ready MRSA. Results were compared to the CHROMagar MRSA results, which was set as reference system. MRSA was detected in 3.05% of the patients with CHROMagar MRSA. LightCycler MRSA Advanced showed a higher clinical sensitivity (84.38%) than Detect-Ready MRSA (57.69%). The negative predictive values were high for both tests (>98%). The specificity and the positive predictive value were higher for the Detect-Ready MRSA test than for the LightCycler MRSA test (99.59% and 78.95% versus 98.52% and 64.29%). For routine screening LightCycler MRSA Advanced proved to be more efficient in our clinical setting as the clinical sensitivity was much higher than the sensitivity of Detect-Ready MRSA. CHROMagar MRSA detected more MRSA positive samples than both PCR methods, leading to the conclusion that the combination of PCR with cultural screening is still the most reliable way for the detection of MRSA. LightCycler MRSA Advanced was faster and needed less hands-on time. The advantage of Detect-Ready MRSA was the additional identification of methicillin-sensitive S. aureus (here in 34.63% of the samples), an information which can be possibly used for reducing the risk of postoperative infections in surgical patients in future.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Aydiner, Aylin UNSPECIFIED UNSPECIFIED UNSPECIFIED Luesebrink, Jessica UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Verena UNSPECIFIED UNSPECIFIED UNSPECIFIED Winterfeld, Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Knuever, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Messler, Sabine UNSPECIFIED UNSPECIFIED UNSPECIFIED Schildgen, Oliver UNSPECIFIED orcid.org/0000-0003-4297-9627 UNSPECIFIED Mattner, Frauke UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-482869
DOI:	10.1371/journal.pone.0043935
Journal or Publication Title:	PLoS One
Volume:	7
Number:	9
Date:	2012
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ASSAY; CHROMOSOME; INFECTIONS; STRAINS; CULTURE; MECA; IDENTIFICATION; COLONIZATION; METAANALYSIS; PREVALENCE Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48286