Universität zu Köln

Lopez-Davila, A. J., Elhamine, Fatiha, Ruess, D. F., Papadopoulos, Simon, Iorga, Bogdan, Kulozik, F. P., Zittrich, Stefan, Solzin, Johannes, Pfitzer, Gabriele and Stehle, Robert (2012). Kinetic Mechanism of Ca2+-controlled Changes of Skeletal Troponin I in Psoas Myofibrils. Biophys. J., 103 (6). S. 1254 - 1265. CAMBRIDGE: CELL PRESS. ISSN 0006-3495

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Abstract

Conformational changes in the skeletal troponin complex (sTn) induced by rapidly increasing or decreasing the [Ca2+] were probed by 5-iodoacetamidofluorescein covalently bound to Cys-133 of skeletal troponin I (sTnI). Kinetics of conformational changes was determined for the isolated complex and after incorporating the complex into rabbit psoas myofibrils. Isolated and incorporated sTn exhibited biphasic Ca2+-activation kinetics. Whereas the fast phase (k(obs)similar to 1000 s(-1)) is only observed in this study, where kinetics were induced by Ca2+, the slower phase resembles the monophasic kinetics of sTnI switching observed in another study (Brenner and Chalovich. 1999. Biophys. J. 77:2692-2708) that investigated the sTnI switching induced by releasing the feedback of force-generating cross-bridges on thin filament activation. Therefore, the slower conformational change likely reflects the sTnI switch that regulates force development. Modeling reveals that the fast conformational change can occur after the first Ca2+ ion binds to skeletal troponin C (sTnC), whereas the slower change requires Ca2+ binding to both regulatory sites of sTnC. Incorporating sTn into myofibrils increased the off-rate and lowered the Ca2+ sensitivity of sTnI switching. Comparison of switch-off kinetics with myofibril force relaxation kinetics measured in a mechanical setup indicates that sTnI switching might limit the rate of fast skeletal muscle relaxation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lopez-Davila, A. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Elhamine, Fatiha UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruess, D. F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Papadopoulos, Simon UNSPECIFIED UNSPECIFIED UNSPECIFIED Iorga, Bogdan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kulozik, F. P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zittrich, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Solzin, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfitzer, Gabriele UNSPECIFIED UNSPECIFIED UNSPECIFIED Stehle, Robert UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-482879
DOI:	10.1016/j.bpj.2012.08.022
Journal or Publication Title:	Biophys. J.
Volume:	103
Number:	6
Page Range:	S. 1254 - 1265
Date:	2012
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	0006-3495
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REGULATORY UNIT INTERACTIONS; RESONANCE ENERGY-TRANSFER; THIN-FILAMENT; MUSCLE-CONTRACTION; STRUCTURAL-CHANGES; CARDIAC TROPONIN; CALCIUM-BINDING; COOPERATIVE BINDING; RELAXATION KINETICS; SKINNED FIBERS Multiple languages Biophysics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48287