Universität zu Köln

Thevis, Mario, Thomas, Andreas ORCID: 0000-0003-1199-0743 and Schaenzer, Wilhelm (2011). Doping control analysis of selected peptide hormones using LC-MS(/MS). Forensic Sci.Int., 213 (1-3). S. 35 - 42. CLARE: ELSEVIER IRELAND LTD. ISSN 1872-6283

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Abstract

With the constantly increasing sensitivity and robustness of liquid chromatography-mass spectrometry-based instruments combined with enhanced reproducibility as well as mass accuracy and resolution, LC-MS(/MS) has become an integral part of sports drug testing programs particularly concerning the detection of peptide hormones. Although several of the relevant peptidic drugs such as insulins (Humalog LisPro, Novolog Aspart, etc.), growth hormone releasing peptides (GHRPs, e. g., GHRP-2, GHRP-6, Hexarelin, etc.), and insulin-like growth factors (e. g., IGF-1, IGF-2, long-R3-IGF-1) are currently analyzed using dedicated top-down analytical procedures, i.e. employing specifically tailored sample preparation procedures followed by targeted LC-MS(/MS) measurements focusing on intact analytes, first approaches towards multi-analyte methods have been established. These allow the determination of the prohibited substances in blood and urine doping control specimens following therapeutic applications. In addition, the use of new complementary devices such as ion mobility analyzers, e. g., in hybrid mass spectrometers yielded promising data for the differentiation of isobaric insulins, which outlines the potential to further accelerate and multiplex doping control analytical assays to meet the continuously increasing demands of rapid and unambiguous test methods. Moreover, the potential of LC-MS/MS to target recombinant peptide hormones such as human growth hormone using bottom-up approaches has been demonstrated by targeting proteotypic peptides that unambiguously differentiate the recombinant molecule from the naturally occurring and endogenously produced analog. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-483556
DOI:	10.1016/j.forsciint.2011.06.015
Journal or Publication Title:	Forensic Sci.Int.
Volume:	213
Number:	1-3
Page Range:	S. 35 - 42
Date:	2011
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1872-6283
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GROWTH-FACTOR-I; MASS-SPECTROMETRIC IDENTIFICATION; HUMAN URINE; LIQUID-CHROMATOGRAPHY; DEGRADATION-PRODUCTS; IGF-I; INSULIN; ABUSE; ANALOGS; PLASMA Multiple languages Medicine, Legal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48355