Universität zu Köln

Beier, A. F. J., Schulz, J. C., Doerr, D., Katsen-Globa, A., Sachinidis, A., Hescheler, J. and Zimmermann, H. (2011). Effective surface-based cryopreservation of human embryonic stem cells by vitrification. Cryobiology, 63 (3). S. 175 - 186. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 1090-2392

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Abstract

Human embryonic stem cells (hESCs) are candidates for many applications in the areas of regenerative medicine, tissue engineering, basic scientific research as well as pharmacology and toxicology. However, use of hESCs is limited by their sensitivity to freezing and thawing procedures. Hence, this emerging science needs new, reliable preservation methods for the long-term storage of large quantities of functional hESCs remaining pluripotent after post-thawing and culturing. Here, we present a highly efficient, surface based vitrification method for the cryopreservation of large numbers of adherent hESC colonies, using modified cell culture substrates. This technique results in much better post-thaw survival rate compared to cryopreservation in suspension and allows a quick and precise handling and storage of the cells, indicating low differentiation rates. (C) 2011 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Beier, A. F. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulz, J. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Doerr, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Katsen-Globa, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Sachinidis, A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zimmermann, H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-484267
DOI:	10.1016/j.cryobiol.2011.06.003
Journal or Publication Title:	Cryobiology
Volume:	63
Number:	3
Page Range:	S. 175 - 186
Date:	2011
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	1090-2392
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INTRACELLULAR ICE; PLURIPOTENCY; PRESERVATION; PROPAGATION; VIABILITY; APOPTOSIS; THERAPY; OOCYTES; INTACT Multiple languages Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48426