Universität zu Köln

Ochrombel, Ines, Becker, Markus, Kraemer, Reinhard and Marin, Kay (2011). Osmotic stress response in C. glutamicum: impact of channel- and transporter-mediated potassium accumulation. Arch. Microbiol., 193 (11). S. 787 - 797. NEW YORK: SPRINGER. ISSN 0302-8933

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Abstract

Potassium accumulation is an essential aspect of bacterial response to diverse stress situations; consequently its uptake plays a pivotal role. Here, we show that the Gram-positive soil bacterium Corynebacterium glutamicum which is employed for the large-scale industrial production of amino acids requires potassium under conditions of ionic and non-ionic osmotic stress. Besides the accumulation of high concentrations of potassium contributing significantly to the osmotic potential of the cytoplasm, we demonstrate that glutamate is not the counter ion for potassium under these conditions. Interestingly, potassium is required for the activation of osmotic stress-dependent expression of the genes betP and proP. The Kup-type potassium transport system which is present in C. glutamicum in addition to the potassium channel CglK does not contribute to potassium uptake at conditions of hyperosmotic stress. Furthermore, we established a secondary carrier of the KtrAB type from C. jeikeium in C. glutamicum thus providing an experimental comparison of channel-and carrier-mediated potassium uptake under osmotic stress. While at low potassium availability, the presence of the KtrAB transporter improves both potassium accumulation and growth of C. glutamicum upon osmotic stress, at proper potassium supply, the channel CglK is sufficient.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ochrombel, Ines UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Kraemer, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Marin, Kay UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-486004
DOI:	10.1007/s00203-011-0717-6
Journal or Publication Title:	Arch. Microbiol.
Volume:	193
Number:	11
Page Range:	S. 787 - 797
Date:	2011
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	0302-8933
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language K+-UPTAKE SYSTEM; CORYNEBACTERIUM-GLUTAMICUM; ESCHERICHIA-COLI; HYPEROSMOTIC STRESS; 2-COMPONENT SYSTEM; COMPATIBLE SOLUTES; BACILLUS-SUBTILIS; BETAINE CARRIER; PH; ADAPTATION Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48600