Universität zu Köln

Bilkovski, R., Schulte, D. M., Oberhauser, F., Mauer, J., Hampel, B., Gutschow, C., Krone, W. and Laudes, M. (2011). Adipose tissue macrophages inhibit adipogenesis of mesenchymal precursor cells via wnt-5a in humans. Int. J. Obes., 35 (11). S. 1450 - 1455. LONDON: NATURE PUBLISHING GROUP. ISSN 0307-0565

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Abstract

In patients with obesity and type 2 diabetes, adipose tissue is infiltrated by macrophages known to alter adipogenesis of mesenchymal precursor cells via secretion of proinflammatory cytokines. Recently, it has been shown that under certain conditions, immune cells can also express wnt-5a, a factor known to inhibit adipogenesis in humans. Therefore, in this study we aimed to investigate whether macrophages affect adipogenesis of mesenchymal precursor cells via wnt-5a. Wnt-5a was found to be expressed in adipose tissue macrophages in obese and type 2 diabetic human subjects in vivo by immunohistochemistry of adipose tissue biopsies. Furthermore, wnt-5a was detectable in circulating CD14(+) blood monocytes of human subjects with obesity and type 2 diabetes on RNA level by real-time PCR. Besides expression analysis in vivo, we also performed functional studies to explore the role of wnt-5a in low-grade inflammation of adipose tissue. In a cell culture experiment, macrophage-conditioned differentiation medium inhibited adipogenesis of 3T3-L1 cells. This inhibitory effect was restored by adding neutralising anti-wnt-5a antibodies. In conclusion, our data indicate that macrophages alter adipogenesis of 3T3-L1 cells not only via classical proinflammatory cytokines, but also via wnt signalling molecules. International Journal of Obesity (2011) 35, 1450-1454; doi:10.1038/ijo.2011.6; published online 1 February 2011

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Bilkovski, R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schulte, D. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Oberhauser, F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mauer, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hampel, B. UNSPECIFIED UNSPECIFIED UNSPECIFIED Gutschow, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Krone, W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Laudes, M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-486079
DOI:	10.1038/ijo.2011.6
Journal or Publication Title:	Int. J. Obes.
Volume:	35
Number:	11
Page Range:	S. 1450 - 1455
Date:	2011
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	0307-0565
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language INSULIN-RESISTANCE; BLOOD MONOCYTES; OBESITY; MECHANISMS Multiple languages Endocrinology & Metabolism; Nutrition & Dietetics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48607