Tomas, C., Axler-DiPerte, G., Budimlija, Z. M., Borsting, C., Coble, M. D., Decker, A. E., Eisenberg, A., Fang, R., Fondevila, M., Fredslund, S. Frisk, Gonzalez, S., Hansen, A. J., Hoff-Olsen, P., Haas, C., Kohler, P., Kriegel, A. K., Lindblom, B., Manohar, F., Maronas, O., Mogensen, H. S., Neureuther, K., Nilsson, H., Scheible, M. K., Schneider, P. M., Sonntag, M. L., Stangegaard, M., Syndercombe-Court, D., Thacker, C. R., Vallone, P. M., Westen, A. A. and Morling, N. (2011). Autosomal SNP typing of forensic samples with the GenPlex (TM) HID System: Results of a collaborative study. Forensic Sci. Int.-Genet., 5 (5). S. 369 - 376. CLARE: ELSEVIER IRELAND LTD. ISSN 1872-4973

Full text not available from this repository.

Abstract

The GenPlex (TM) HID System (Applied Biosystems - AB) offers typing of 48 of the 52 SNPforID SNPs and amelogenin. Previous studies have shown a high reproducibility of the GenPlex (TM) HID System using 250500 pg DNA of good quality. An international exercise was performed by 14 laboratories (9 in Europe and 5 in the US) in order to test the robustness and reliability of the GenPlex (TM) HID System on forensic samples. Three samples with partly degraded DNA and 10 samples with low amounts of DNA were analyzed in duplicates using various amounts of DNA. In order to compare the performance of the GenPlex (TM) HID System with the most commonly used STR kits, 500 pg of partly degraded DNA from three samples was typed by the laboratories using one or more STR kits. The median SNP typing success rate was 92.3% with 500 pg of partly degraded DNA. Three of the fourteen laboratories counted for more than two thirds of the locus dropouts. The median percentage of discrepant results was 0.2% with 500 pg degraded DNA. An increasing percentage of locus dropouts and discrepant results were observed when lower amounts of DNA were used. Different success rates were observed for the various SNPs. The rs763869 SNP was the least successful. With the exception of the MiniFiler (TM) kit (AB), GenPlex (TM) HID performed better than five other tested STR kits. When partly degraded DNA was analyzed, GenPlex (TM) HID showed a very low mean mach probability, while all STR kits except MiniFiler (TM) had very limited discriminatory power. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Tomas, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Axler-DiPerte, G.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Budimlija, Z. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Borsting, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Coble, M. D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Decker, A. E.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Eisenberg, A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fang, R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fondevila, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fredslund, S. FriskUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gonzalez, S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hansen, A. J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hoff-Olsen, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Haas, C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kohler, P.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kriegel, A. K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lindblom, B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Manohar, F.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maronas, O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mogensen, H. S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Neureuther, K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nilsson, H.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Scheible, M. K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Sonntag, M. L.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stangegaard, M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Syndercombe-Court, D.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thacker, C. R.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vallone, P. M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Westen, A. A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Morling, N.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-486888
DOI: 10.1016/j.fsigen.2010.06.007
Journal or Publication Title: Forensic Sci. Int.-Genet.
Volume: 5
Number: 5
Page Range: S. 369 - 376
Date: 2011
Publisher: ELSEVIER IRELAND LTD
Place of Publication: CLARE
ISSN: 1872-4973
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SINGLE-NUCLEOTIDE POLYMORPHISMS; ASSAYMultiple languages
Genetics & Heredity; Medicine, LegalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/48688

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item