Universität zu Köln

Ladage, D., Turnbull, I. C., Ishikawa, K., Takewa, Y., Rapti, K., Morel, C., Karakikes, I., Hadri, L., Mueller-Ehmsen, J., Costa, K. D., Hajjar, R. J. and Kawase, Y. (2011). Delivery of gelfoam-enabled cells and vectors into the pericardial space using a percutaneous approach in a porcine model. Gene Ther., 18 (10). S. 979 - 986. LONDON: NATURE PUBLISHING GROUP. ISSN 0969-7128

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Abstract

Intrapericardial drug delivery is a promising procedure, with the ability to localize therapeutics with the heart. Gelfoam particles are nontoxic, inexpensive, nonimmunogenic and biodegradable compounds that can be used to deliver therapeutic agents. We developed a new percutaneous approach method for intrapericardial injection, puncturing the pericardial sac safely under fluoroscopy and intravascular ultrasound (IVUS) guidance. In a porcine model of myocardial infarction (MI), we deployed gelfoam particles carrying either (a) autologous mesenchymal stem cells (MSCs) or (b) an adenovirus encoding enhanced green fluorescent protein (eGFP) 48 h post-MI. The presence of MSCs and viral infection at the infarct zone was confirmed by immunoflourescence and PCR. Puncture was performed successfully in 16 animals. Using IVUS, we successfully determined the size of the pericardial space before the puncture, and safely accessed that space in setting of pericardial effusion and also adhesions induced by the MI. Intrapericardial injection of gelfoam was safe and reliable. Presence of the MSCs and eGFP expression from adenovirus in the myocardium were confirmed after delivery. Our novel percutaneous approach to deliver (stem-) cells or adenovirus was safe and efficient in this pre-clinical model. IVUS-guided delivery is a minimally invasive procedure that seems to be a promising new strategy to deliver therapeutic agents locally to the heart. Gene Therapy (2011) 18, 979-985; doi:10.1038/gt.2011.52; published online 21 April 2011

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ladage, D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Turnbull, I. C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ishikawa, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Takewa, Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED Rapti, K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Morel, C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Karakikes, I. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hadri, L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller-Ehmsen, J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Costa, K. D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hajjar, R. J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kawase, Y. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-487805
DOI:	10.1038/gt.2011.52
Journal or Publication Title:	Gene Ther.
Volume:	18
Number:	10
Page Range:	S. 979 - 986
Date:	2011
Publisher:	NATURE PUBLISHING GROUP
Place of Publication:	LONDON
ISSN:	0969-7128
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MESENCHYMAL STEM-CELLS; MYOCARDIAL ANGIOGENESIS; EPICARDIAL DEPOSITION; DRUG-DELIVERY; GENE-TRANSFER; MARROW; GELATIN; INFARCTION; IMPLANTATION; INJECTION Multiple languages Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48780