Universität zu Köln

Lupu, Marilena, Khalil, Markus, Iordache, Florin ORCID: 0000-0002-5366-6792, Andrei, Eugen, Pfannkuche, Kurt, Spitkovsky, Dimitry, Baumgartner, Sven, Rubach, Martin, AbdelRazik, Heba, Buzila, Cosmin, Brockmeier, Konrad, Simionescu, Maya, Hescheler, Juergen and Maniu, Horia (2011). Direct contact of umbilical cord blood endothelial progenitors with living cardiac tissue is a requirement for vascular tube-like structures formation. J. Cell. Mol. Med., 15 (9). S. 1914 - 1927. HOBOKEN: WILEY. ISSN 1582-4934

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Abstract

The umbilical cord blood derived endothelial progenitor cells (EPCs) contribute to vascular regeneration in experimental models of ischaemia. However, their ability to participate in cardiovascular tissue restoration has not been elucidated yet. We employed a novel coculture system to investigate whether human EPCs have the capacity to integrate into living and ischaemic cardiac tissue, and participate to neovascularization. EPCs were cocultured with either living or ischaemic murine embryonic ventricular slices, in the presence or absence of a pro-angiogenic growth factor cocktail consisting of VEGF, IGF-1, EGF and bFGF. Tracking of EPCs within the cocultures was performed by cell transfection with green fluorescent protein or by immunostaining performed with anti-human vWF, CD31, nuclei and mitochondria antibodies. EPCs generated vascular tube-like structures in direct contact with the living ventricular slices. Furthermore, the pro-angiogenic growth factor cocktail reduced significantly tubes formation. Coculture of EPCs with the living ventricular slices in a transwell system did not lead to vascular tube-like structures formation, demonstrating that the direct contact is necessary and that the soluble factors secreted by the living slices were not sufficient for their induction. No vascular tubes were formed when EPCs were cocultured with ischaemic ventricular slices, even in the presence of the pro-angiogenic cocktail. In conclusion, EPCs form vascular tube-like structures in contact with living cardiac tissue and the direct cell-to-cell interaction is a prerequisite for their induction. Understanding the cardiac niche and micro-environmental interactions that regulate EPCs integration and neovascularization are essential for applying these cells to cardiovascular regeneration.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Lupu, Marilena UNSPECIFIED UNSPECIFIED UNSPECIFIED Khalil, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Iordache, Florin UNSPECIFIED orcid.org/0000-0002-5366-6792 UNSPECIFIED Andrei, Eugen UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfannkuche, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Spitkovsky, Dimitry UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumgartner, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Rubach, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED AbdelRazik, Heba UNSPECIFIED UNSPECIFIED UNSPECIFIED Buzila, Cosmin UNSPECIFIED UNSPECIFIED UNSPECIFIED Brockmeier, Konrad UNSPECIFIED UNSPECIFIED UNSPECIFIED Simionescu, Maya UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Maniu, Horia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-489775
DOI:	10.1111/j.1582-4934.2010.01197.x
Journal or Publication Title:	J. Cell. Mol. Med.
Volume:	15
Number:	9
Page Range:	S. 1914 - 1927
Date:	2011
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1582-4934
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ACUTE MYOCARDIAL-INFARCTION; BONE-MARROW-CELLS; IN-VITRO MODEL; STEM-CELLS; MATRIX-METALLOPROTEINASE; GROWTH-FACTOR; EXPRESSION; HEART; TRANSPLANTATION; ANGIOGENESIS Multiple languages Cell Biology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48977