Universität zu Köln

Nicholson, Scott J., Hoecker, Ute and Srivastava, Vibha ORCID: 0000-0002-3031-6984 (2011). A novel Phytochrome B allele in Arabidopsis thaliana exhibits partial mutant phenotype: a short deletion in N-terminal extension reduces Phytochrome B activity. Plant Growth Regul., 65 (1). S. 207 - 213. DORDRECHT: SPRINGER. ISSN 0167-6903

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Abstract

During analysis of an Arabidopsis thaliana line possessing a Phytochrome A epiallele (phyA'), a partial Phytochrome B-deficient phenotype was observed, consisting of elongated hypocotyls in seedlings grown under constant white light or red light (660 nm). The observed hypocotyls were twice the length (8 mm) of wild-type (4 mm), but approximately half the length of a null phyB-9 mutant (14 mm). Several analyses were performed to characterize this apparent partial phyB mutant. Sequencing of the entire exonic region revealed three point mutations that altered codon usage, and one in-frame 12 base pair deletion. Each of the point mutations has been described in other lines that display wild-type phenotype, and therefore their effect is thought to be minimal, if any. The N-terminal deletion of amino acids 9 through 12 (GGGR) is a unique mutation found in this line. This deletion most likely contributes to the phyB mutant phenotype by lowering the binding affinity of the active form of Phytochrome B (Pfr) with Phytochrome Interacting Factor 3 (PIF3).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Nicholson, Scott J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED UNSPECIFIED UNSPECIFIED Srivastava, Vibha UNSPECIFIED orcid.org/0000-0002-3031-6984 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-489849
DOI:	10.1007/s10725-011-9576-z
Journal or Publication Title:	Plant Growth Regul.
Volume:	65
Number:	1
Page Range:	S. 207 - 213
Date:	2011
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	0167-6903
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHYSIOLOGICAL-RESPONSES; SIGNAL-TRANSDUCTION; FUNCTIONAL-ANALYSIS; DNA METHYLATION; LIGHT; GENE; PIF3; EXPRESSION; MUTATIONS; DOMAIN Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48984