Universität zu Köln

Kuenzel, Timo, Heiermann, Reinhard, Frank, Uri ORCID: 0000-0003-2094-6381, Mueller, Werner, Tilmann, Wido, Bause, Markus ORCID: 0000-0002-5172-2631, Nonn, Anja, Helling, Matthias, Schwarz, Ryan S. and Plickert, Guenter (2010). Migration and differentiation potential of stem cells in the cnidarian Hydractinia analysed in eGFP-transgenic animals and chimeras. Dev. Biol., 348 (1). S. 120 - 130. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 0012-1606

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Abstract

To analyse cell migration and the differentiation potential of migratory stem cells in Hydractinia, we generated animals with an eGFP reporter gene stably expressed and transmitted via the germline. The transgene was placed under the control of two different actin promoters and the promoter of elongation factor-1 alpha, One actin promoter (Act-II) and the EF-1 alpha promoter enabled expression of the transgene in all cells, the other actin promoter (Act-I) in epithelial and gametogenic cells, but not in the pluripotent migratory stem cells. We produced chimeric animals consisting of histocompatible wild type and transgenic parts. When the transgene was under the control of the epithelial cell specific actin-I promoter, non-fluorescent transgenic stem cells immigrated into wild type tissue, stopped migration and differentiated into epithelial cells which then commenced eGFP-expression. Migratory stem cells are therefore pluripotent and can give rise not only to germ cells, nematocytes and nerve cells, but also to epithelial cells. While in somatic cells expression of the act-I promoter was restricted to epithelial cells it became also active in gametogenesis. The act-I gene is expressed in spermatogonia, oogonia and oocytes. In males the expression pattern showed that migratory stem cells are the precursors of both the spermatogonia and their somatic envelopes. Comparative expression studies using the promoters of the actin-II gene and the elongation factor-1 alpha gene revealed the potential of transgenic techniques to trace the development of the nervous system. (C) 2010 Elsevier Inc. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kuenzel, Timo UNSPECIFIED UNSPECIFIED UNSPECIFIED Heiermann, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Frank, Uri UNSPECIFIED orcid.org/0000-0003-2094-6381 UNSPECIFIED Mueller, Werner UNSPECIFIED UNSPECIFIED UNSPECIFIED Tilmann, Wido UNSPECIFIED UNSPECIFIED UNSPECIFIED Bause, Markus UNSPECIFIED orcid.org/0000-0002-5172-2631 UNSPECIFIED Nonn, Anja UNSPECIFIED UNSPECIFIED UNSPECIFIED Helling, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Schwarz, Ryan S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plickert, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-491329
DOI:	10.1016/j.ydbio.2010.08.017
Journal or Publication Title:	Dev. Biol.
Volume:	348
Number:	1
Page Range:	S. 120 - 130
Date:	2010
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication:	SAN DIEGO
ISSN:	0012-1606
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HYDROID HYDRACTINIA; ECHINATA; EXPRESSION; EVOLUTION; GENE; ALLORECOGNITION; ONTOGENY; HISTOINCOMPATIBILITY; SPERMATOGENESIS; PROLIFERATION Multiple languages Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49132