Universität zu Köln

Schroers, Roland, Baraniskin, Alexander, Heute, Christoph, Vorgerd, Matthias, Brunn, Anna, Kuhnhenn, Jan, Kowoll, Annika, Alekseyev, Andriy, Schmiegel, Wolff, Schlegel, Uwe, Deckert, Martina and Pels, Hendrik (2010). Diagnosis of leptomeningeal disease in diffuse large B-cell lymphomas of the central nervous system by flow cytometry and cytopathology. Eur. J. Haematol., 85 (6). S. 520 - 529. HOBOKEN: WILEY. ISSN 1600-0609

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Abstract

Reliable detection of leptomeningeal disease has the potential of facilitating the diagnosis of central nervous system (CNS) lymphoma and is important for therapeutic considerations. Currently, the standard diagnostic procedure for the detection of lymphoma in the cerebrospinal fluid is cytopathology. To improve the limited specificity and sensitivity of cytopathology, flow cytometry has been suggested as an alternative. Here, we evaluated multi-parameter flow cytometry in combination with conventional cytopathology in cerebrospinal fluid (CSF) samples from 30 patients with primary CNS lymphoma and seven patients with secondary CNS lymphoma. Overall, in 11 of 37 (29.7%) patients with CNS lymphoma, lymphoma cells were detected in CSF by flow cytometry, while cytopathology was less sensitive displaying unequivocally malignant CSF cells in only seven of all 37 (18.9%) patients. Six (16.2%) patients showed cytopathological results suspicious of lymphoma; however, in only one of these patients, the diagnosis of CSF lymphoma cells could be confirmed by flow cytometry. In primary CNS lymphomas (PCNSL), seven of 30 (23.3%) patients were positive for CSF lymphoma cells in flow cytometry, in contrast to four (13.3%) patients with PCNSL with definitely positive cytopathology. In summary, our results suggest that multi-parameter flow cytometry increases the sensitivity and specificity of leptomeningeal disease detection in CNS lymphomas. Both methods should be applied concurrently for complementary diagnostic assessment in patients with CNS lymphoma.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schroers, Roland UNSPECIFIED UNSPECIFIED UNSPECIFIED Baraniskin, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Heute, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Vorgerd, Matthias UNSPECIFIED UNSPECIFIED UNSPECIFIED Brunn, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuhnhenn, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Kowoll, Annika UNSPECIFIED UNSPECIFIED UNSPECIFIED Alekseyev, Andriy UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmiegel, Wolff UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlegel, Uwe UNSPECIFIED UNSPECIFIED UNSPECIFIED Deckert, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Pels, Hendrik UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-491398
DOI:	10.1111/j.1600-0609.2010.01516.x
Journal or Publication Title:	Eur. J. Haematol.
Volume:	85
Number:	6
Page Range:	S. 520 - 529
Date:	2010
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1600-0609
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PRIMARY CNS LYMPHOMA; NON-HODGKINS-LYMPHOMA; CEREBROSPINAL-FLUID; MENINGEAL DISSEMINATION; IDENTIFICATION; SENSITIVITY Multiple languages Hematology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49139