Universität zu Köln

Fritsche-Guenther, Raphaela, Gruetzkau, Andreas, Noske, Aurelia, Melcher, Ingo, Schaser, Klaus-Dieter, Schlag, Peter M., Kasper, Hans-Udo, Krenn, Veit and Sers, Christine (2010). Therapeutic potential of CAMPATH-1H in skeletal tumours. Histopathology, 57 (6). S. 851 - 862. MALDEN: WILEY-BLACKWELL PUBLISHING, INC. ISSN 0309-0167

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Abstract

Therapeutic potential of CAMPATH-1H in skeletal tumours Aims: CD 52 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that is expressed abundantly on all lymphocytes, monocytes, macrophages, eosinophils and in the male genital tract. To date, the physiological role of CD52 on lymphocytes has not been elucidated. However, an antibody directed to CD52 called CAMPATH-1H has been shown to be capable of depleting lymphocytes. The aim of this study was to analyse tissue and cell lines of non-neoplastic bone, cartilage and skeletal tumours for CD52 expression. Methods and results: The expression of CD52 mRNA and protein both in vivo and in vitro was detected. Malignant tumours showed higher CD52 expression compared to benign tumours, suggesting a role in the development and progression of bone tumours. Interestingly, immunohistochemistry and flow cytometry revealed that CD52 was expressed not only on the surface of tumour cells, but also in the cytoplasm. The results obtained in osteosarcoma cells showed that CAMPATH-1H leads to a complement-independent reduction of viable cells. Conclusion: CD52 is expressed in a variety of bone tumours and the in vitro studies presented herein suggest that CAMPATH-1H treatment might have therapeutic potential for osteosarcoma patients.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fritsche-Guenther, Raphaela UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruetzkau, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Noske, Aurelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Melcher, Ingo UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaser, Klaus-Dieter UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlag, Peter M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kasper, Hans-Udo UNSPECIFIED UNSPECIFIED UNSPECIFIED Krenn, Veit UNSPECIFIED UNSPECIFIED UNSPECIFIED Sers, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-491517
DOI:	10.1111/j.1365-2559.2010.03722.x
Journal or Publication Title:	Histopathology
Volume:	57
Number:	6
Page Range:	S. 851 - 862
Date:	2010
Publisher:	WILEY-BLACKWELL PUBLISHING, INC
Place of Publication:	MALDEN
ISSN:	0309-0167
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHRONIC LYMPHOCYTIC-LEUKEMIA; COMPLEMENT-MEDIATED LYSIS; VERSUS-HOST-DISEASE; ANTIBODY CAMPATH-1; CD52; ANTIGEN; DEPLETION; TRANSPLANTATION; ALEMTUZUMAB; EXPRESSION Multiple languages Cell Biology; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49151