Universität zu Köln

Venkatesh, G. Raja, Koungni, Frant Carlot Kembou, Paukner, Andreas, Stratmann, Thomas ORCID: 0000-0002-9843-6384, Blissenbach, Birgit and Schnetz, Karin ORCID: 0000-0003-4693-6202 (2010). BglJ-RcsB Heterodimers Relieve Repression of the Escherichia coli bgl Operon by H-NS. J. Bacteriol., 192 (24). S. 6456 - 6465. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5530

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Abstract

RcsB is the response regulator of the complex Rcs two-component system, which senses perturbations in the outer membrane and peptidoglycan layer. BglJ is a transcriptional regulator whose constitutive expression causes activation of the H-NS- and StpA-repressed bgl (aryl-beta, D-glucoside) operon in Escherichia coli. RcsB and BglJ both belong to the LuxR-type family of transcriptional regulators with a characteristic C-terminal DNA-binding domain. Here, we show that BglJ and RcsB interact and form heterodimers that presumably bind upstream of the bgl promoter, as suggested by mutation of a sequence motif related to the consensus sequence for RcsA-RcsB heterodimers. Heterodimerization of BglJ-RcsB and relief of H-NS-mediated repression of bgl by BglJ-RcsB are apparently independent of RcsB phosphorylation. In addition, we show that LeuO, a pleiotropic LysR-type transcriptional regulator, likewise binds to the bgl upstream regulatory region and relieves repression of bgl independently of BglJ-RcsB. Thus, LeuO can affect bgl directly, as shown here, and indirectly by activating the H-NS-repressed yjjQ-bglJ operon, as shown previously. Taken together, heterodimer formation of RcsB and BglJ expands the role of the Rcs two-component system and the network of regulators affecting the bgl promoter.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Venkatesh, G. Raja UNSPECIFIED UNSPECIFIED UNSPECIFIED Koungni, Frant Carlot Kembou UNSPECIFIED UNSPECIFIED UNSPECIFIED Paukner, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Stratmann, Thomas UNSPECIFIED orcid.org/0000-0002-9843-6384 UNSPECIFIED Blissenbach, Birgit UNSPECIFIED UNSPECIFIED UNSPECIFIED Schnetz, Karin UNSPECIFIED orcid.org/0000-0003-4693-6202 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-491581
DOI:	10.1128/JB.00807-10
Journal or Publication Title:	J. Bacteriol.
Volume:	192
Number:	24
Page Range:	S. 6456 - 6465
Date:	2010
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5530
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MEDIATED REPRESSION; GENE-EXPRESSION; STRESS-RESPONSE; TRANSCRIPTION; PROMOTER; LEUO; DNA; PHOSPHORELAY; BINDING; SYSTEM Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49158