Universität zu Köln

Zeng, Jia and Howard, Jonathan C. (2010). Spontaneous focal activation of invariant natural killer T ( iNKT) cells in mouse liver and kidney. BMC Biol., 8. LONDON: BMC. ISSN 1741-7007

Full text not available from this repository.

Abstract

Background: Invariant natural killer T (iNKT) cells differ from other T cells by their hyperactive effector T-cell status, in addition to the expression of NK lineage receptors and semi-invariant T-cell receptors. It is generally agreed that the immune phenotype of iNKT cells is maintained by repeated activation in peripheral tissues although no explicit evidence for such iNKT cell activity in vivo has so far been reported. Results: We used an interferon (IFN)-gamma-inducible cytoplasmic protein, Irga6, as a histological marker for local IFN-gamma production. Irga6 was intensely expressed in small foci of liver parenchymal cells and kidney tubular epithelium. Focal Irga6 expression was unaffected by germ-free status or loss of TLR signalling and was totally dependent on IFN-gamma secreted by T cells in the centres of expression foci. These were shown to be iNKT cells by diagnostic T cell receptor usage and their activity was lost in both CD1 d and J alpha-deficient mice. Conclusions: This is the first report that supplies direct evidence for explicit activation events of NKT cells in vivo and raises issues about the triggering mechanism and consequences for immune functions in liver and kidney.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zeng, Jia UNSPECIFIED UNSPECIFIED UNSPECIFIED Howard, Jonathan C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-492179
DOI:	10.1186/1741-7007-8-142
Journal or Publication Title:	BMC Biol.
Volume:	8
Date:	2010
Publisher:	BMC
Place of Publication:	LONDON
ISSN:	1741-7007
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RECEPTOR-ALPHA-CHAIN; NKT CELLS; TARGETED DISRUPTION; MUTANT MICE; IFN-GAMMA; GENE; GLOMERULONEPHRITIS; HEPATOCYTES; REPERTOIRES; LYMPHOCYTES Multiple languages Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49217