Universität zu Köln

Michel, Sven, Liang, Liming, Depner, Martin, Klopp, Norman, Ruether, Andreas, Kumar, Ashish ORCID: 0000-0002-7075-5930, Schedel, Michaela ORCID: 0000-0001-8465-2986, Vogelberg, Christian, von Mutius, Erika ORCID: 0000-0002-8893-4515, von Berg, Andrea, Bufe, Albrecht ORCID: 0000-0001-7335-9362, Rietschel, Ernst, Heinzmann, Andrea, Laub, Otto, Simma, Burkhard, Frischer, Thomas, Genuneit, Jon, Gut, Ivo G., Schreiber, Stefan, Lathrop, Mark, Illig, Thomas and Kabesch, Michael (2010). Unifying Candidate Gene and GWAS Approaches in Asthma. PLoS One, 5 (11). SAN FRANCISCO: PUBLIC LIBRARY SCIENCE. ISSN 1932-6203

Full text not available from this repository.

Abstract

The first genome wide association study (GWAS) for childhood asthma identified a novel major susceptibility locus on chromosome 17q21 harboring the ORMDL3 gene, but the role of previous asthma candidate genes was not specifically analyzed in this GWAS. We systematically identified 89 SNPs in 14 candidate genes previously associated with asthma in >3 independent study populations. We re-genotyped 39 SNPs in these genes not covered by GWAS performed in 703 asthmatics and 658 reference children. Genotyping data were compared to imputation data derived from Illumina HumanHap300 chip genotyping. Results were combined to analyze 566 SNPs covering all 14 candidate gene loci. Genotyped polymorphisms in ADAM33, GSTP1 and VDR showed effects with p-values <0.0035 (corrected for multiple testing). Combining genotyping and imputation, polymorphisms in DPP10, EDN1, IL12B, IL13, IL4, IL4R and TNF showed associations at a significance level between p = 0.05 and p = 0.0035. These data indicate that (a) GWAS coverage is insufficient for many asthma candidate genes, (b) imputation based on these data is reliable but incomplete, and (c) SNPs in three previously identified asthma candidate genes replicate in our GWAS population with significance after correction for multiple testing in 14 genes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Michel, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Liang, Liming UNSPECIFIED UNSPECIFIED UNSPECIFIED Depner, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Klopp, Norman UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruether, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kumar, Ashish UNSPECIFIED orcid.org/0000-0002-7075-5930 UNSPECIFIED Schedel, Michaela UNSPECIFIED orcid.org/0000-0001-8465-2986 UNSPECIFIED Vogelberg, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED von Mutius, Erika UNSPECIFIED orcid.org/0000-0002-8893-4515 UNSPECIFIED von Berg, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Bufe, Albrecht UNSPECIFIED orcid.org/0000-0001-7335-9362 UNSPECIFIED Rietschel, Ernst UNSPECIFIED UNSPECIFIED UNSPECIFIED Heinzmann, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Laub, Otto UNSPECIFIED UNSPECIFIED UNSPECIFIED Simma, Burkhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Frischer, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Genuneit, Jon UNSPECIFIED UNSPECIFIED UNSPECIFIED Gut, Ivo G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Schreiber, Stefan UNSPECIFIED UNSPECIFIED UNSPECIFIED Lathrop, Mark UNSPECIFIED UNSPECIFIED UNSPECIFIED Illig, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Kabesch, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-492527
DOI:	10.1371/journal.pone.0013894
Journal or Publication Title:	PLoS One
Volume:	5
Number:	11
Date:	2010
Publisher:	PUBLIC LIBRARY SCIENCE
Place of Publication:	SAN FRANCISCO
ISSN:	1932-6203
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NECROSIS-FACTOR-ALPHA; ETHNICALLY DIVERSE POPULATIONS; GENOME-WIDE ASSOCIATION; CHILDHOOD ASTHMA; BRONCHIAL HYPERRESPONSIVENESS; RECEPTOR POLYMORPHISMS; ADAM33 POLYMORPHISMS; ATOPIC ASTHMA; ADULT ASTHMA; BIRTH COHORT Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49252