Universität zu Köln

Ghozzi, Stephane ORCID: 0000-0002-3911-9573, Ng, Jerome Wong, Chatenay, Didier and Robert, Jerome (2010). Inference of plasmid-copy-number mean and noise from single-cell gene expression data. Phys. Rev. E, 82 (5). COLLEGE PK: AMER PHYSICAL SOC. ISSN 1550-2376

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Abstract

Plasmids are extrachromosomal DNA molecules which code for their own replication. We previously reported a setup using genes coding for fluorescent proteins of two colors that allowed us, using a simple model, to extract the plasmid-copy-number noise in a monoclonal population of bacteria [J. Wong Ng et al., Phys. Rev. E 81, 011909 (2010)]. Here we present a detailed calculation relating this noise to the measured levels of fluorescence, taking into account all sources of fluorescence fluctuations: not only the fluctuation of gene expression as in the simple model but also the growth and division of bacteria, the nonuniform distribution of their ages, the random partition of proteins at divisions, and the replication and partition of plasmids and chromosome. We show how to use the chromosome as a reference, which helps extracting the plasmid-copy-number noise in a self-consistent manner.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ghozzi, Stephane UNSPECIFIED orcid.org/0000-0002-3911-9573 UNSPECIFIED Ng, Jerome Wong UNSPECIFIED UNSPECIFIED UNSPECIFIED Chatenay, Didier UNSPECIFIED UNSPECIFIED UNSPECIFIED Robert, Jerome UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-492558
DOI:	10.1103/PhysRevE.82.051916
Journal or Publication Title:	Phys. Rev. E
Volume:	82
Number:	5
Date:	2010
Publisher:	AMER PHYSICAL SOC
Place of Publication:	COLLEGE PK
ISSN:	1550-2376
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CHROMOSOME; DYNAMICS; REPLICATION Multiple languages Physics, Fluids & Plasmas; Physics, Mathematical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49255