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Boerngen, Kirsten, Battle, Andrew R., Moeker, Nina, Morbach, Susanne, Marin, Kay, Martinac, Boris ORCID: 0000-0001-8422-7082 and Kraemer, Reinhard (2010). The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation. Biochim. Biophys. Acta-Biomembr., 1798 (11). S. 2141 - 2150. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 0006-3002

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Abstract

Based on sequence similarity, the mscCG gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to investigate the physiological significance of MscCG in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an Escherichia coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating a strongly rectifying behavior. Apart from that. MscCG is characterized by significant functional differences with respect to conductance, ion selectivity and desensitation behavior as compared to MscS from E. coli. Deletion and complementation studies in C glutamicum showed a significant contribution of MscCG to betaine efflux in response to hypoosmotic conditions. A detailed analysis of concomitant betaine uptake (by the betaine transporter BetP) and efflux (by MscCG) under hyperosmotic conditions indicates that MscCG may act in osmoregulation in C. glutamicum by fine-tuning the steady state concentration of compatible solutes in the cytoplasm which are accumulated in response to hyperosmotic stress. (C) 2010 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Boerngen, Kirsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Battle, Andrew R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Moeker, Nina UNSPECIFIED UNSPECIFIED UNSPECIFIED Morbach, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED Marin, Kay UNSPECIFIED UNSPECIFIED UNSPECIFIED Martinac, Boris UNSPECIFIED orcid.org/0000-0001-8422-7082 UNSPECIFIED Kraemer, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-493018
DOI:	10.1016/j.bbamem.2010.06.022
Journal or Publication Title:	Biochim. Biophys. Acta-Biomembr.
Volume:	1798
Number:	11
Page Range:	S. 2141 - 2150
Date:	2010
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	0006-3002
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MECHANOSENSITIVE ION CHANNELS; ESCHERICHIA-COLI MSCS; BETAINE CARRIER BETP; BIOCHEMICAL-CHARACTERIZATION; IDENTIFICATION; CONDUCTION; PRESSURE; SYSTEM; CELLS Multiple languages Biochemistry & Molecular Biology; Biophysics Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49301