Universität zu Köln

Montzka, Katrin, Fuehrmann, Tobias, Mueller-Ehmsen, Jochen, Woeltje, Michael and Brook, Gary A. (2010). Growth factor and cytokine expression of human mesenchymal stromal cells is not altered in an in vitro model of tissue damage. Cytotherapy, 12 (7). S. 870 - 881. OXFORD: ELSEVIER SCI LTD. ISSN 1477-2566

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Abstract

Background aims. The beneficial effect of human (h) mesenchymal stromal cell (MSC) transplantation in a variety of cell-based intervention strategies is widely believed to be because of paracrine mechanisms. The modification of hMSC cytokine and growth-factor expression patterns were studied following exposure to lipopolysaccharide (LPS) and tissue homogenates (representing tissue debris) from normal and pathologic tissues. Methods. Human bone marrow-derived MSC were stimulated with LPS or exposed to homogenate from normal or pathologic rat spinal cord or heart. The expression profiles of a number of cytokines and growth factors were investigated using quantitative reverse transcription (RT)-polymerase chain reaction (PCR) with human-specific primers. The effects of tissue homogenates on hMSC proliferation and migratory behavior were also investigated. Results. Stimulation of hMSC with LPS resulted in an up-regulation of interleukin (IL)-1 beta, IL-6 and IL-8. However, the pattern of up-regulation varied between donor samples. Furthermore, LPS treatment resulted in a donor-dependent alteration of growth factor expression. Induction of a shift in expression pattern was not observed following exposure to homogenates from either normal or pathologic tissues. Tissue homogenates did stimulate cell proliferation, but not migration. Conclusions. The hMSC expression pattern is apparently stable, even when cells are confronted by debris from different tissue types. However, treatment of hMSC with LPS is able to change the expression of cytokines and growth factors in a donor-dependent manner that may enhance their potential use in regenerative medicine.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Montzka, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Fuehrmann, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller-Ehmsen, Jochen UNSPECIFIED UNSPECIFIED UNSPECIFIED Woeltje, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Brook, Gary A. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-493117
DOI:	10.3109/14653249.2010.501789
Journal or Publication Title:	Cytotherapy
Volume:	12
Number:	7
Page Range:	S. 870 - 881
Date:	2010
Publisher:	ELSEVIER SCI LTD
Place of Publication:	OXFORD
ISSN:	1477-2566
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SPINAL-CORD-INJURY; BONE-MARROW-CELLS; RECEPTOR C-MET; STEM-CELLS; FUNCTIONAL RECOVERY; MYOCARDIAL-INFARCTION; NEUROTROPHIC FACTOR; ENDOTHELIAL-CELLS; AXON GROWTH; RAT MODEL Multiple languages Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell Biology; Hematology; Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49311