Universität zu Köln

Woo, Han Min ORCID: 0000-0002-8797-0477, Noack, Stephan ORCID: 0000-0001-9784-3626, Seibold, Gerd M., Willbold, Sabine ORCID: 0000-0002-8335-9478, Eikmanns, Bernhard J. and Bott, Michael ORCID: 0000-0002-4701-8254 (2010). Link between Phosphate Starvation and Glycogen Metabolism in Corynebacterium glutamicum, Revealed by Metabolomics. Appl. Environ. Microbiol., 76 (20). S. 6910 - 6920. WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-5336

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Abstract

In this study, we analyzed the influence of phosphate (P-i) limitation on the metabolism of Corynebacterium glutamicum. Metabolite analysis by gas chromatography-time-of-flight (GC-TOF) mass spectrometry of cells cultivated in glucose minimal medium revealed a greatly increased maltose level under P-i limitation. As maltose formation could be linked to glycogen metabolism, the cellular glycogen content was determined. Unlike in cells grown under P-i excess, the glycogen level in P-i-limited cells remained high in the stationary phase. Surprisingly, even acetate-grown cells, which do not form glycogen under P-i excess, did so under P-i limitation and also retained it in stationary phase. Expression of pgm and glgC, encoding the first two enzymes of glycogen synthesis, phosphoglucomutase and ADP-glucose pyrophosphorylase, was found to be increased 6- and 3-fold under P-i limitation, respectively. Increased glycogen synthesis together with a decreased glycogen degradation might be responsible for the altered glycogen metabolism. Independent from these experimental results, flux balance analysis suggested that an increased carbon flux to glycogen is a solution for C. glutamicum to adapt carbon metabolism to limited P-i concentrations.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Woo, Han Min UNSPECIFIED orcid.org/0000-0002-8797-0477 UNSPECIFIED Noack, Stephan UNSPECIFIED orcid.org/0000-0001-9784-3626 UNSPECIFIED Seibold, Gerd M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Willbold, Sabine UNSPECIFIED orcid.org/0000-0002-8335-9478 UNSPECIFIED Eikmanns, Bernhard J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bott, Michael UNSPECIFIED orcid.org/0000-0002-4701-8254 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-494626
DOI:	10.1128/AEM.01375-10
Journal or Publication Title:	Appl. Environ. Microbiol.
Volume:	76
Number:	20
Page Range:	S. 6910 - 6920
Date:	2010
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-5336
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ESCHERICHIA-COLI; REGULATOR SUGR; EXPRESSION; TREHALOSE; SYSTEM; GROWTH; DEHYDROGENASE; MALTODEXTRIN; ENCODES; MODELS Multiple languages Biotechnology & Applied Microbiology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49462