Universität zu Köln

Luan, Shi-Lu, Boulanger, Emmanuelle, Ye, Hongtao, Chanudet, Estelle, Johnson, Nicola, Hamoudi, Rifat A., Bacon, Chris M., Liu, Hongxiang, Huang, Yuanxue, Said, Jonathan, Chu, Peiguo, Clemen, Christoph S., Cesarman, Ethel, Chadburn, Amy, Isaacson, Peter G. and Du, Ming-Qing ORCID: 0000-0002-1017-5045 (2010). Primary effusion lymphoma: genomic profiling revealed amplification of SELPLG and CORO1C encoding for proteins important for cell migration. J. Pathol., 222 (2). S. 166 - 180. HOBOKEN: WILEY. ISSN 1096-9896

Full text not available from this repository.

Abstract

Primary effusion lymphoma (PEL) is associated with Kaposi sarcoma herpesvirus (KSHV) but its pathogenesis is poorly understood. Many KSHV-associated products can deregulate cellular pathways commonly targeted in cancer. However, KSHV infection alone is insufficient for malignant transformation. PEL also lacks the chromosomal translocations seen in other lymphoma subtypes. We investigated 28 PELs and ten PEL cell lines by 1 Mb resolution array comparative genomic hybridization (CGH) and found frequent gains of 1q21-41 (47%), 4q28.3-35 (29%), 7q (58%), 8q (63%), 11 (32%), 12 (61%), 17q (29%), 19p (34%), and 20q (34%), and losses of 4q (32%), 11q25 (29%), and 14q32 (63%). Recurrent focal amplification was seen at several regions on chromosomes 7, 8, and 12. High-resolution chromosome-specific tile-path array CGH confirmed these findings, and identified selectin-P ligand (SELPLG) and coronin-1C (CORO1C) as the targets of a cryptic amplification at 12q24.11. Interphase FISH and quantitative PCR showed SELPLG/CORO1C amplification (>4 extra copies) and low levels of copy number gain (1-4 extra copies) in 23% of PELs, respectively. Immunohistochemistry revealed strong expression of both SELPLG and coronin-1C in the majority of PELs, irrespective of their gene dosage. SELPLG is critical for cell migration and chemotaxis, while CORO1C regulates actin-dependent processes, thus important for cell motility. Their overexpression in PEL is expected to play an important role in its pathogenesis. Copyright (C) 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Luan, Shi-Lu UNSPECIFIED UNSPECIFIED UNSPECIFIED Boulanger, Emmanuelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Ye, Hongtao UNSPECIFIED UNSPECIFIED UNSPECIFIED Chanudet, Estelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Johnson, Nicola UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamoudi, Rifat A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Bacon, Chris M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Hongxiang UNSPECIFIED UNSPECIFIED UNSPECIFIED Huang, Yuanxue UNSPECIFIED UNSPECIFIED UNSPECIFIED Said, Jonathan UNSPECIFIED UNSPECIFIED UNSPECIFIED Chu, Peiguo UNSPECIFIED UNSPECIFIED UNSPECIFIED Clemen, Christoph S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cesarman, Ethel UNSPECIFIED UNSPECIFIED UNSPECIFIED Chadburn, Amy UNSPECIFIED UNSPECIFIED UNSPECIFIED Isaacson, Peter G. UNSPECIFIED UNSPECIFIED UNSPECIFIED Du, Ming-Qing UNSPECIFIED orcid.org/0000-0002-1017-5045 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-495857
DOI:	10.1002/path.2752
Journal or Publication Title:	J. Pathol.
Volume:	222
Number:	2
Page Range:	S. 166 - 180
Date:	2010
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1096-9896
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SARCOMA-ASSOCIATED HERPESVIRUS; MULTICENTRIC CASTLEMANS-DISEASE; KAPOSIS-SARCOMA; NUCLEAR ANTIGEN; SOLID LYMPHOMAS; GENE-EXPRESSION; MALT LYMPHOMA; HUMAN-HERPESVIRUS-8; VIRUS; P53 Multiple languages Oncology; Pathology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49585