Universität zu Köln

Sonje, Marina Bubonja, Abram, Maja ORCID: 0000-0003-4113-098X, Stenzel, Werner ORCID: 0000-0002-1143-2103 and Deckert, Martina (2010). Listeria monocytogenes (delta-actA mutant) infection in tumor necrosis factor receptor p55-deficient neonatal mice. Microb. Pathog., 49 (4). S. 186 - 196. LONDON: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD. ISSN 0882-4010

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Abstract

Using TNF receptor 1 knock out (TNFR1KO) mice, we investigated the role played by TNFR1 in immune regulation during neonatal listeriosis. Induction of protective immune response in wild type pups resulted in the prompt control of infection with an attenuated Delta actA mutant Listeria monocytogenes, accompanied by enhanced hepatic expression of mRNA for IFN-gamma, INF-alpha, and IL-10 Conversely, the lack of TNFR1 signalling in TNFR I KO neonatal mice resulted in substantial changes in the profile of inflammatory mediators and ultimately fatal outcome of the infected pups Despite remarkable increase in indoleamine 2, 3-dioxygenase (IDO) and inducible nitric oxide synthase (NOS) mRNA detected in the liver of TNFR1KO mice, bacterial proliferation was unrestrained Increased mRNA expression of IDO, iNOS, TNF-alpha, IFN-gamma, MCP-1, and MIP-1 alpha was found in the spleens of infected KO mice, and in the brains mRNA encoding iNOS, IDO, IFN-gamma, IL-12p40, IL-10. and RANTES was also upregulated. Large necrotic lesions consisting of granulocytes and macrophages were scattered throughout the liver of these mice. TNFR1KO neonates were unable to clear neutrophils and switch from the innate immune response to a specific reaction mediated by T cells. These results prove that TNF-alpha signalling is crucial and irreplaceable in antilisterial protection during the neonatal period. (C) 2010 Elsevier Ltd. All rights reserved

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sonje, Marina Bubonja UNSPECIFIED UNSPECIFIED UNSPECIFIED Abram, Maja UNSPECIFIED orcid.org/0000-0003-4113-098X UNSPECIFIED Stenzel, Werner UNSPECIFIED orcid.org/0000-0002-1143-2103 UNSPECIFIED Deckert, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-495979
DOI:	10.1016/j.micpath.2010.05.012
Journal or Publication Title:	Microb. Pathog.
Volume:	49
Number:	4
Page Range:	S. 186 - 196
Date:	2010
Publisher:	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Place of Publication:	LONDON
ISSN:	0882-4010
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TNF-ALPHA; INDOLEAMINE 2,3-DIOXYGENASE; BACTERIAL-INFECTION; ENDOTOXIC-SHOCK; INNATE IMMUNITY; IFN-GAMMA; INDUCTION; RESPONSES; RESISTANT; LACKING Multiple languages Immunology; Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49597