Universität zu Köln

Duffy, David J., Plickert, Guenter, Kuenzel, Timo, Tilmann, Wido and Frank, Uri ORCID: 0000-0003-2094-6381 (2010). Wnt signaling promotes oral but suppresses aboral structures in Hydractinia metamorphosis and regeneration. Development, 137 (18). S. 3057 - 3067. CAMBRIDGE: COMPANY OF BIOLOGISTS LTD. ISSN 0950-1991

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Abstract

We studied the role of Wnt signaling in axis formation during metamorphosis and regeneration in the cnidarian Hydractinia. Activation of Wnt downstream events during metamorphosis resulted in a complete oralization of the animals and repression of aboral structures (i.e. stolons). The expression of Wnt3, Tcf and Brachyury was upregulated and became ubiquitous. Rescue experiments using Tcf RNAi resulted in normal metamorphosis and quantitatively normal Wnt3 and Brachyury expression. Isolated, decapitated polyps regenerated only heads but no stolons. Activation of Wnt downstream targets in regenerating animals resulted in oralization of the polyps. Knocking down Tcf or Wnt3 by RNAi inhibited head regeneration and resulted in complex phenotypes that included ectopic aboral structures. Multiple heads then grew when the RNAi effect had dissipated. Our results provide functional evidence that Wnt promotes head formation but represses the formation of stolons, whereas downregulation of Wnt promotes stolons and represses head formation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Duffy, David J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Plickert, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuenzel, Timo UNSPECIFIED UNSPECIFIED UNSPECIFIED Tilmann, Wido UNSPECIFIED UNSPECIFIED UNSPECIFIED Frank, Uri UNSPECIFIED orcid.org/0000-0003-2094-6381 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496566
DOI:	10.1242/dev.046631
Journal or Publication Title:	Development
Volume:	137
Number:	18
Page Range:	S. 3057 - 3067
Date:	2010
Publisher:	COMPANY OF BIOLOGISTS LTD
Place of Publication:	CAMBRIDGE
ISSN:	0950-1991
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CNIDARIAN CLYTIA-HEMISPHAERICA; HYDRA HEAD REGENERATION; PRIMARY BODY AXIS; BETA-CATENIN; PLANARIAN REGENERATION; STEM-CELLS; POLARITY; ECHINATA; NEUROPEPTIDES; EVOLUTION Multiple languages Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49656