Universität zu Köln

von Brandenstein, Melanie, Herden, Jan, Koditz, Barbara, Huerta, Manuel, Nestler, Tim, Heidenreich, Axel and Fries, Jochen W. U. (2021). Non-invasive urine markers for the differentiation between RCCs and oncocytoma. J. Clin. Lab. Anal., 35 (5). HOBOKEN: WILEY. ISSN 1098-2825

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Abstract

Background Recently, our group showed that Vim3 is overexpressed in tissue samples of renal oncocytomas and Mxi-2 in clear cell renal carcinoma (ccRCC). The mechanism leading to the truncation of both proteins is known and involves with two miRs, both detectable in urine. Since the analysis of miRs is time-consuming, our aim was to identify the truncated proteins in urine instead. Furthermore, urine samples from small renal masses (SRMs) (n = 45, <4 cm) were analyzed to get a pre-surgical differentiation of the cancer subtypes. Methods Urines were accessed from the urological biobank (n = 350). Proteins were isolated from urine samples, and Western blots were performed. Each sample was analyzed with ELISA for the expression of Vim3 and Mxi-2. A lateral flow assay was established. For the detection of SRMs, the miRs were isolated and qRT-PCR was performed. Results A significant increase of Vim3 in urines from patients with oncocytoma (n = 20) was detectable with ELISA compared to all other subtypes of RCCs (chromophobe (n = 50), papillary (n = 40), ccRCC (n = 200), and controls (n = 40) (***p < 0.0001)). Mxi-2 was predominantly overexpressed in ccRCCs (***p < 0.0001). Lateral flow assay of Vim3 and Mxi-2 shows two bands in the case of oncocytoma and ccRCC indicating the specificity of this test. For SRMs, an overexpression of miR-15a/Mxi2 was detectable in urine samples from ccRCC and chromoRCC patients. In contrast to that, miR-498/Vim3 were predominantly overexpressed in oncocytoma patients. Conclusion Both proteins (Vim3 and Mxi-2) were detectable in patients' urines and can be used for the non-invasive differentiation of kidney cancers.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code von Brandenstein, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Herden, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Koditz, Barbara UNSPECIFIED UNSPECIFIED UNSPECIFIED Huerta, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Nestler, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Heidenreich, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED Fries, Jochen W. U. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-568416
DOI:	10.1002/jcla.23762
Journal or Publication Title:	J. Clin. Lab. Anal.
Volume:	35
Number:	5
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1098-2825
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Medical Laboratory Technology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56841