Universität zu Köln

Hribek, Agathe, Mestanoglu, Mert, Clahsen, Thomas, Reinking, Niklas, Frentzen, Falk, Howaldt, Antonia ORCID: 0000-0001-7596-8073, Siebelmann, Sebastian, Bachmann, Bjoern O., Cursiefen, Claus and Matthaei, Mario (2022). Scheimpflug Backscatter Imaging of the Fibrillar Layer in Fuchs Endothelial Corneal Dystrophy. Am. J. Ophthalmol., 235. S. 63 - 71. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1879-1891

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Abstract

Purpose: A central collagen-rich subendothelial fibrillar layer (FL) correlates with areas of accentuated loss of corneal endothelial cells in advanced Fuchs endothelial corneal dystrophy (FECD). The present study sought to investigate whether the FL may be visualized by en face Scheimpflug backscatter imaging in vivo. Design: Retrospective analysis of a prospective observational case series. Methods: A total of 34 eyes (34 subjects) undergoing Descemet membrane endothelial keratoplasty (DMEK) surgery with preoperative high-quality Scheimpflug backscatter imaging data were included. The Descemet endothelium complex (DEC) was retrieved during DMEK surgery, and immunofluorescence staining was performed for collagens I, III, and IV. The FL morphology in en face Scheimpflug backscatter and immunofluorescence imaging was compared and agreement of FL parameters was analyzed using intraclass correlation coefficients (ICC) and Bland-Altman plots. Results: Scheimpflug backscatter imaging delineated the FL in 26 eyes and was FL negative in 8 eyes with deviation compared to immunofluorescence in 1 case and good agreement of morphology characteristics. Horizontical caliper diameter +/- SD was 4.84 +/- 0.85 mm, vertical caliper diameter was 3.92 +/- 0.78 mm, maximum caliper diameter was 5.12 +/- 0.82 mm, and surface area was 12.43 +/- 4.74 mm(2). Compared to immunofluorescence imaging, mean difference (95% limits of agreement) and intraclass correlation coefficients were for horizontal caliper diameter 0.13 mm (-0.81 to 1.1 mm) and 0.88, vertical caliper diameter 0.23 mm (-0.76 to 1.2 mm) and 0.81, maximum caliper diameter 0.06 mm (-1.1 to 1.2 mm) and 0.86, and surface area 1.4 mm(2) (-3.9 to 6.7 mm(2)) and 0.85. Conclusions: Scheimpflug backscatter imaging facilitates visualization of the FL in advanced FECD eyes, offering the potential to identify particularly diseased areas of the FECD endothelium in vivo. ((C) 2021 Elsevier Inc. All rights reserved.)

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hribek, Agathe UNSPECIFIED UNSPECIFIED UNSPECIFIED Mestanoglu, Mert UNSPECIFIED UNSPECIFIED UNSPECIFIED Clahsen, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Reinking, Niklas UNSPECIFIED UNSPECIFIED UNSPECIFIED Frentzen, Falk UNSPECIFIED UNSPECIFIED UNSPECIFIED Howaldt, Antonia UNSPECIFIED orcid.org/0000-0001-7596-8073 UNSPECIFIED Siebelmann, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Bachmann, Bjoern O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cursiefen, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Matthaei, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-569714
DOI:	10.1016/j.ajo.2021.08.019
Journal or Publication Title:	Am. J. Ophthalmol.
Volume:	235
Page Range:	S. 63 - 71
Date:	2022
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1879-1891
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXTRACELLULAR-MATRIX ALTERATIONS; MEMBRANE; TRANSPLANTATION; DENSITOMETRY; KERATOPLASTY Multiple languages Ophthalmology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/56971