Universität zu Köln

Frerigmann, Henning, Hoecker, Ute ORCID: 0000-0002-5636-9777 and Gigolashvili, Tamara (2021). New Insights on the Regulation of Glucosinolate Biosynthesis via COP1 and DELLA Proteins in Arabidopsis Thaliana. Front. Plant Sci., 12. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-462X

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Abstract

The biosynthesis of defensive secondary metabolites, such as glucosinolates (GSLs), is a costly process, which requires nutrients, ATP, and reduction equivalents, and, therefore, needs well-orchestrated machinery while coordinating defense and growth. We discovered that the key repressor of light signaling, the CONSTITUTIVE PHOTOMORPHOGENIC 1/SUPPRESSOR OF PHYTOCHROME A-105 (COP1/SPA) complex, is a crucial component of GSL biosynthesis regulation. Various mutants in this COP1/SPA complex exhibited a strongly reduced level of GSL and a low expression of jasmonate (JA)-dependent genes. Furthermore, cop1, which is known to accumulate DELLA proteins in the dark, shows reduced gibberellin (GA) and JA signaling, thereby phenocopying other DELLA-accumulating mutants. This phenotype can be complemented by a dominant gain-of-function allele of MYC3 and by crossing with a mutant having low DELLA protein levels. Hence, SPA1 interacts with DELLA proteins in a yeast two-hybrid screen, whereas high levels of DELLA inhibit MYC function and suppress JA signaling. DELLA accumulation leads to reduced synthesis of GSL and inhibited growth. Thus, the COP1/SPA-mediated degradation of DELLA not only affects growth but also regulates the biosynthesis of GSLs.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Frerigmann, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Hoecker, Ute UNSPECIFIED orcid.org/0000-0002-5636-9777 UNSPECIFIED Gigolashvili, Tamara UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-570925
DOI:	10.3389/fpls.2021.680255
Journal or Publication Title:	Front. Plant Sci.
Volume:	12
Date:	2021
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-462X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MYB TRANSCRIPTION FACTORS; UBIQUITIN LIGASE COP1/SPA; SEED-GERMINATION; BOTRYTIS-CINEREA; LIGHT REGULATION; PLANT-RESPONSES; JAZ REPRESSORS; GROWTH; DEFENSE; MYC2 Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57092