Universität zu Köln

Murad, Fabronia and Garcia-Saez, Ana J. (2021). Bcl-xL inhibits tBid and Bax via distinct mechanisms. Faraday Discuss., 232. S. 86 - 103. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 1364-5498

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Abstract

The proteins of the Bcl-2 family are key regulators of apoptosis. They form a complex interaction network in the cytosol and in cellular membranes, whose outcome determines mitochondrial permeabilization and commitment to death. However, we still do not understand how the action of the different family members is orchestrated to regulate apoptosis. Here, we combined quantitative analysis of the interactions and the localization dynamics of the family representatives Bcl-xL, Bax and tBid, in living cells. We discovered that Bax and tBid are able to constitutively shuttle between cytosol and mitochondria in the absence of other Bcl-2 proteins. Bcl-xL clearly stabilized tBid at mitochondria, where they formed tight complexes. In contrast, Bcl-xL promoted Bax retrotranslocation to the cytosol without affecting its shuttling rate, but by forming weak inhibitory mitochondrial complexes. Furthermore, analysis of phospho-mimetics of Bcl-xL suggested that phosphorylation regulates the function of Bcl-xL via multiple mechanisms. Altogether, our findings support a model in which the Bcl-2 network not only modulates protein/protein interactions among the family members, but also their respective intracellular localization dynamics, to regulate apoptosis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Murad, Fabronia UNSPECIFIED UNSPECIFIED UNSPECIFIED Garcia-Saez, Ana J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-572841
DOI:	10.1039/d0fd00045k
Journal or Publication Title:	Faraday Discuss.
Volume:	232
Number:	0
Page Range:	S. 86 - 103
Date:	2021
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	1364-5498
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL-DEATH; PHOSPHORYLATION SITE; MEMBRANE DYNAMICS; PROTEIN FAMILY; APOPTOSIS; BCL-X(L); KINASE; MITOCHONDRIA; IDENTIFICATION; RETROTRANSLOCATION Multiple languages Chemistry, Physical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57284