Universität zu Köln

Ye, Lijun ORCID: 0000-0002-9760-9263, Ji, Haichao, Liu, Jie, Tu, Chien-Hua, Kappl, Michael, Koynov, Kaloian ORCID: 0000-0002-4062-8834, Vogt, Johannes ORCID: 0000-0003-2439-8247 and Butt, Hans-Juergen (2021). Carbon Nanotube-Hydrogel Composites Facilitate Neuronal Differentiation While Maintaining Homeostasis of Network Activity. Adv. Mater., 33 (41). WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-4095

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Abstract

It is often assumed that carbon nanotubes (CNTs) stimulate neuronal differentiation by transferring electrical signals and enhancing neuronal excitability. Given this, CNT-hydrogel composites are regarded as potential materials able to combine high electrical conductivity with biocompatibility, and therefore promote nerve regeneration. However, whether CNT-hydrogel composites actually influence neuronal differentiation and maturation, and how they do so remain elusive. In this study, CNT-hydrogel composites are prepared by in situ polymerization of poly(ethylene glycol) around a preformed CNT meshwork. It is demonstrated that the composites facilitate long-term survival and differentiation of pheochromocytoma 12 cells. Adult neural stem cells cultured on the composites show an increased neuron-to-astrocyte ratio and higher synaptic connectivity. Moreover, primary hippocampal neurons cultured on composites maintain morphological synaptic features as well as their neuronal network activity evaluated by spontaneous calcium oscillations, which are comparable to neurons cultured under control conditions. These results indicate that the composites are promising materials that could indeed facilitate neuronal differentiation while maintaining neuronal homeostasis.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ye, Lijun UNSPECIFIED orcid.org/0000-0002-9760-9263 UNSPECIFIED Ji, Haichao UNSPECIFIED UNSPECIFIED UNSPECIFIED Liu, Jie UNSPECIFIED UNSPECIFIED UNSPECIFIED Tu, Chien-Hua UNSPECIFIED UNSPECIFIED UNSPECIFIED Kappl, Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Koynov, Kaloian UNSPECIFIED orcid.org/0000-0002-4062-8834 UNSPECIFIED Vogt, Johannes UNSPECIFIED orcid.org/0000-0003-2439-8247 UNSPECIFIED Butt, Hans-Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-572929
DOI:	10.1002/adma.202102981
Journal or Publication Title:	Adv. Mater.
Volume:	33
Number:	41
Date:	2021
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-4095
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language STEM-CELLS; PLASTICITY; CALCIUM; STIMULATION; EXTENSION; OUTGROWTH Multiple languages Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57292