Universität zu Köln

Cox, Alexander ORCID: 0000-0001-7837-5740, Tolkach, Yuri, Stein, Johannes ORCID: 0000-0002-6316-1229, Kristiansen, Glen, Ritter, Manuel and Ellinger, Jorg (2021). Otoferlin is a prognostic biomarker in patients with clear cell renal cell carcinoma: A systematic expression analysis. Int. J. Urol., 28 (4). S. 424 - 432. HOBOKEN: WILEY. ISSN 1442-2042

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Abstract

Objectives: To comprehensively investigate the role of otoferlin as a prognostic and diagnostic biomarker in clear cell renal cell carcinoma. Methods: Three independent cohorts were used to study otoferlin in clear cell renal cell carcinoma: The Cancer Genome Atlas cohort (messenger ribonucleic acid expression; clear cell renal cell carcinoma n = 514, normal renal tissue n = 81); study validation cohort (messenger ribonucleic acid expression; clear cell renal cell carcinoma n = 79, normal renal tissue n = 44); and immunohistochemistry cohort (protein expression; clear cell renal cell carcinoma n = 142, normal renal tissue n = 30). Otoferlin gene expressions were extracted from The Cancer Genome Atlas database or determined using quantitative real-time polymerase chain reaction, respectively. Protein expression was assessed using immunohistochemistry staining against otoferlin on tissue microarrays. Correlations between otoferlin messenger ribonucleic acid/protein expression and clinicopathological data/patient survival were statistically tested. Results: Otoferlin messenger ribonucleic acid expression was significantly upregulated in clear cell renal cell carcinoma compared with normal renal tissue. High expression levels correlated with advanced stage, higher grade and metastatic tumors, accompanied by independent prognostic significance for overall and cancer-specific survival. In contrast, otoferlin protein expression was downregulated in tumor tissue. Although, high otoferlin expression in clear cell renal cell carcinoma was positively correlated with histological grading and independently predictive of a shortened progression-free survival. Conclusion: Our data suggest otoferlin as an indicator of tumor aggressiveness and as a prognostic biomarker for patients with clear cell renal cell carcinoma, leading to the conclusion that otoferlin could promote the malignancy of clear cell renal cell carcinoma.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cox, Alexander UNSPECIFIED orcid.org/0000-0001-7837-5740 UNSPECIFIED Tolkach, Yuri UNSPECIFIED UNSPECIFIED UNSPECIFIED Stein, Johannes UNSPECIFIED orcid.org/0000-0002-6316-1229 UNSPECIFIED Kristiansen, Glen UNSPECIFIED UNSPECIFIED UNSPECIFIED Ritter, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Ellinger, Jorg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-574181
DOI:	10.1111/iju.14486
Journal or Publication Title:	Int. J. Urol.
Volume:	28
Number:	4
Page Range:	S. 424 - 432
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1442-2042
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Urology & Nephrology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57418